Ordering Recommendation

PCR testing is preferred for diagnosis of acute infection. Detect neutralizing antibodies to coxsackie B virus.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST) or Plain Red.

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens.

Storage/Transport Temperature


Unacceptable Conditions

CSF or plasma. Contaminated, hemolyzed, or severely lipemic specimens.


Mark specimens plainly as "acute" or "convalescent."


Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Serum Neutralization




6-12 days

Reference Interval

Coxsackie B1: Less than 1:10
Coxsackie B2: Less than 1:10
Coxsackie B3: Less than 1:10
Coxsackie B4: Less than 1:10
Coxsackie B5: Less than 1:10
Coxsackie B6: Less than 1:10

Interpretive Data

Single positive antibody titers of greater than or equal to 1:80 may indicate past or current infection.  Seroconversion or an increase in titers between acute and convalescent sera of at least fourfold is considered strong evidence of current or recent infection.

Compliance Category



Hotline History


CPT Codes

86658 x6


Component Test Code* Component Chart Name LOINC
0065111 Coxsackie B Virus Antibody Type 1 5103-7
0065112 Coxsackie B Virus Antibody Type 2 5105-2
0065113 Coxsackie B Virus Antibody Type 3 5107-8
0065123 Coxsackie B Virus Antibody Type 5 5111-0
0065124 Coxsackie B Virus Antibody Type 6 5113-6
0065130 Coxsackie B Virus Antibody Type 4 5109-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Coxsackie Antibody, Neutralization
  • Coxsackie B
  • Coxsackie B Antibody Panel-Serum
  • Coxsackie B virus antibody profile, IgG, IgM
  • Enterovirus
Coxsackie B Virus Antibodies