Ordering Recommendation
Test detects the Shiga toxins, sensitive markers of toxigenic Escherichia coli. CDC recommends stool specimens also be cultured for E. coli O157:H7 (O157 STEC).
Mnemonic
Methodology
Qualitative Enzyme Immunoassay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
Specimen Required
Stool. OR actively growing isolate of E. coli, in pure culture.
Stool: Place 5 mL stool in enteric transport media (Cary-Blair) (ARUP Supply #29799) immediately after collection. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 1 mL)
E. coli Isolate: Transport sealed container with pure culture on agar slant or on a swab in bacterial transport media. Place each specimen in an individually sealed bag.
Stool: Refrigerated.
E. coli Isolate: Room temperature.
Specimens or isolates from sources other than stool.
Stool in Transport Media: Ambient: 1 hour; Refrigerated: 72 hours; Frozen: 1 week
E. coli Isolate: Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval
Negative
Interpretive Data
By report.
FDA
Note
This test will identify the presence of E. coli Shiga-like toxin, however it cannot determine specific of strains of E. coli. (e.g., E. coli O157:H7)
Hotline History
CPT Codes
87427
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0060047 | E. coli Shiga-like Toxin by EIA | 21262-1 |
Aliases
- Ecoli 0157
- EHEC
- Enteropathogenic E. coli
- Escherichia Coli 0157 Stool Antigen Detection
- Shiga-like Toxin, fecal
- Verotoxin Assay