Ordering Recommendation

Test detects the Shiga toxins, sensitive markers of toxigenic Escherichia coli. CDC recommends stool specimens also be cultured for E. coli O157:H7 (O157 STEC).

Mnemonic
ECSLT AG
Methodology

Qualitative Enzyme Immunoassay

Performed

Sun-Sat

Reported

Within 2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Stool. OR actively growing isolate of E. coli, in pure culture.

Specimen Preparation

Stool: Place 5 mL stool in enteric transport media (Cary-Blair) (ARUP Supply #29799) immediately after collection. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 1 mL)
E. coli Isolate:
Transport sealed container with pure culture on agar slant or on a swab in bacterial transport media. Place each specimen in an individually sealed bag.

Storage/Transport Temperature

Stool: Refrigerated.
E. coli
Isolate:
Room temperature.

Unacceptable Conditions

Specimens or isolates from sources other than stool.

Remarks
Stability

Stool in Transport Media: Ambient: 1 hour; Refrigerated: 72 hours; Frozen: 1 week
E. coli
Isolate:
Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data

By report.

No compliance statements are in use for this test.

Note

This test will identify the presence of E. coli Shiga-like toxin, however it cannot determine specific of strains of E. coli. (e.g., E. coli O157:H7)

Hotline History
N/A
CPT Codes

87427

Components
Component Test Code* Component Chart Name LOINC
0060047 E. coli Shiga-like Toxin by EIA 21262-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Ecoli 0157
  • EHEC
  • Enteropathogenic E. coli
  • Escherichia Coli 0157 Stool Antigen Detection
  • Shiga-like Toxin, fecal
  • Verotoxin Assay
E. coli Shiga-like Toxin by EIA