Ordering Recommendation

HIV-1 genotyping provides antiretroviral susceptibility information for protease inhibitors (PI) and reverse transcriptase inhibitors (NRTI, NNRTI). Intended for patients with viral load >1,000 copies/mL.




Reverse Transcription Polymerase Chain Reaction/Sequencing




3-7 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA), pink (K2EDTA) or plasma preparation tube.

Specimen Preparation

Separate plasma from cells within 6 hours. Transfer 4 mL plasma to an ARUP Standard Transport Tube. (Min: 1.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

Serum. Heparinized specimens.


Please submit most recent viral load and test date, if available.


On Cells: Ambient: 6 hours; After separation from cells: Ambient: 24 hours; Refrigerated: 5 days; Frozen: 4 months

Reference Interval

By report

Interpretive Data

This test predicts HIV-1 resistance to protease and reverse transcriptase inhibitor anti-retroviral drugs. The protease gene and codons 1-335 of the reverse transcriptase gene of the viral genome are sequenced using the Viroseq HIV-1 Genotyping System kit. Drug resistance is assigned using ViroSeq software. The most current resistance algorithm and drug list is available by selecting the Drug Resistance Report.

This test should be used in conjunction with clinical presentation and other laboratory markers. A patient's response to therapy depends on multiple factors including patient compliance, percentage of resistant virus population, dosing, and drug pharmacology issues. Resistance interpretations may vary with test methodology.

Some insertions or deletions may be difficult to detect using this software. This test may not detect minor HIV-1 populations less than 20 percent of the total population.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Modified FDA


This test may be unsuccessful if the plasma HIV-1 RNA viral load is less than 1,000 HIV-1 RNA copies per mL of plasma.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0051722 HIV-1 Genotype by Sequencing 53798-5
2002141 EER HIV-1 Genotype by Sequencing 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • HIV Drug Resistance
  • HIV Genotype
  • HIV1 Genotype
  • HIV1 Genotyping
  • HIV1 Protease and Reverse Transcriptase Genotype
  • HIV1 Protease and Reverse Transcriptase Genotyping
  • HIV1 Protease and Reverse Transcriptase Sequencing
  • HIV1 Protease Genotyping
  • HIV1 Reverse Transcriptase Genotyping
  • HIV1 Sequencing
Human Immunodeficiency Virus 1, Genotype by Sequencing (INACTIVE as of 08/16/21: Refer to 3003853 with the August Hotline)