HIV-1 genotyping provides antiretroviral susceptibility information for protease inhibitors (PI) and reverse transcriptase inhibitors (NRTI, NNRTI). Intended for patients with viral load >1,000 copies/mL.
Reverse Transcription Polymerase Chain Reaction/Sequencing
Lavender (EDTA), pink (K2EDTA) or plasma preparation tube.
Separate plasma from cells within 6 hours. Transfer 4 mL plasma to an ARUP Standard Transport Tube. (Min: 1.5 mL)
Serum. Heparinized specimens.
Please submit most recent viral load and test date, if available.
On Cells: Ambient: 6 hours; After separation from cells: Ambient: 24 hours; Refrigerated: 5 days; Frozen: 4 months
This test predicts HIV-1 resistance to protease and reverse transcriptase inhibitor anti-retroviral drugs. The protease gene and codons 1-335 of the reverse transcriptase gene of the viral genome are sequenced using the Viroseq HIV-1 Genotyping System kit. Drug resistance is assigned using ViroSeq software. The most current resistance algorithm and drug list is available by selecting the Drug Resistance Report.
This test should be used in conjunction with clinical presentation and other laboratory markers. A patient's response to therapy depends on multiple factors including patient compliance, percentage of resistant virus population, dosing, and drug pharmacology issues. Resistance interpretations may vary with test methodology.
Some insertions or deletions may be difficult to detect using this software. This test may not detect minor HIV-1 populations less than 20 percent of the total population.
This test may be unsuccessful if the plasma HIV-1 RNA viral load is less than 1,000 HIV-1 RNA copies per mL of plasma.
|Component Test Code*||Component Chart Name||LOINC|
|0051722||HIV-1 Genotype by Sequencing||53798-5|
|2002141||EER HIV-1 Genotype by Sequencing||11526-1|
- HIV Drug Resistance
- HIV Genotype
- HIV1 Genotype
- HIV1 Genotyping
- HIV1 Protease and Reverse Transcriptase Genotype
- HIV1 Protease and Reverse Transcriptase Genotyping
- HIV1 Protease and Reverse Transcriptase Sequencing
- HIV1 Protease Genotyping
- HIV1 Reverse Transcriptase Genotyping
- HIV1 Sequencing