Ordering Recommendation

Not a first-line test for Lyme disease. May be useful if strong suspicion of Lyme disease persists in spite of persistent negative serologic testing. Blood and CSF specimens have poor clinical sensitivity for detection of Borrelia burgdorferi by PCR.




Qualitative Polymerase Chain Reaction


Mon, Wed, Fri


1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR CSF, synovial fluid or tissue.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma, CSF or synovial fluid to a sterile container. (Min: 0.5 mL). Tissue: Transfer to a sterile container and freeze immediately.

Storage/Transport Temperature


Unacceptable Conditions

Heparinized specimens, tissues in optimal cutting temperature compound.


Specimen source required.


Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 year
All Others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 1 year

Reference Interval

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0051048 Borrelia Species Source 31208-2
0055570 Borrelia Species DNA Detection by PCR 4991-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • B. burgdorferi DNA detection
  • Borrelia burgdorferi
  • Lyme Disease
  • Lyme Disease PCR
  • Tick Borne Disease
Borrelia Species by PCR (Lyme Disease)