Aid in the diagnosis of T-cell lymphoproliferative disorders.
Polymerase Chain Reaction/Capillary Electrophoresis
DNA Isolation: Sun-Sat
Assay: Mon, Wed-Fri
Lavender (EDTA), bone marrow (EDTA), or tissue. Also acceptable: DNA extracted by CLIA certified lab.
Whole Blood: Do not freeze. Transport 5 mL. (Min: 1 mL)
Bone marrow: Do not freeze. Transport 3 mL. (Min: 1 mL)
Fresh Tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue
FFPE Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Tissue block will be returned after testing. Transport tissue block or four 10-micron shavings in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40uL DNA with at least 50 ng/uL concentration. Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Whole Blood, Bone Marrow or Extracted DNA: Refrigerated.
Fresh Tissue: Frozen on dry ice.
FFPE Tumor Tissue: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Whole Blood, Bone Marrow or Extracted DNA: Plasma, serum, or DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.
Tissue: Specimens fixed/processed in alternative fixatives, heavy metal fixatives (B-4 or B-5), or tissue sections on slides. Decalcified specimens.
Whole Blood or Bone Marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE Tumor Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|0055567||T-Cell Clonality by PCR||21753-9|
|2006285||T-Cell Clonality Source|
- T Cell Gamma
- T Cell Gene Rearrangement
- T-cell receptor gamma rearrangment
- T-Cell Receptor Gene Rearrangement