Support the diagnosis of immune neutropenia in various autoimmune disorders.
Qualitative Flow Cytometry
Plain red or serum separator tube.
Remove serum from cells ASAP or within 2 hours of collection. Transfer 3 mL serum to an ARUP Standard Transport Tube and freeze. (Min: 0.5 mL)
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month
Neutrophil-associated antibodies may cause neutropenia in various autoimmune disorders including Felty syndrome, SLE and drug-induced neutropenia. Febrile transfusion reactions and isoimmune neonatal neutropenia may also be caused by antibodies to neutrophil-specific antigens or HLA antigens.
A positive result on this test is not definitive for specific antineutrophil antibodies, since anti-HLA antibodies and immune complexes may also cause a positive result. The results of this test should be correlated to clinical history and other data.
Laboratory Developed Test (LDT)
Circulating antibodies in patient's serum are measured by flow cytometry after incubation with normal neutrophils. Values greater than 2 standard deviations of a normal control population are interpreted as "weakly positive" and greater than 3 standard deviations as "positive".
This test should not be confused with Anti-Neutrophil Cytoplasmic Antibody, IgG (0050811 - ANCA).
|Component Test Code*||Component Chart Name||LOINC|
|0055506||Neutrophil Associated Antibodies||41617-2|
- Anti-Granulocyte Antibodies
- Anti-Leukocyte Ab
- Anti-Neutrophil Antibodies
- Granulocyte Ab
- Neutrophil Antibody