Screening test for Lynch Syndrome. Determine potential eligibility for immunotherapy by identifying microsatellite instability-high (MSI-H) cancers.
Polymerase Chain Reaction/Fragment Analysis
DNA isolation: Sun-Sat
Tumor AND normal epithelial tissue. Also acceptable: DNA extracted by CLIA certified
Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block(s) or 10 unstained 5-micron slides (5 tumor and 5 normal epithelial). (Min: 3 tumor tissue and 3 normal epithelial tissue slides) Transport block(s) and/or slide(s) in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL)Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months
Extracted DNA: Refrigerated.
Less than 25 percent tumor or less than 50 percent normal epithelial tissue. DNA extracted by a non-CLIA lab. DNA extracted without a corresponding circled H&E slide. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.
Include surgical pathology report.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerated: Indefinitely; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|0051741||Microsatellite Marker BAT-25|
|0051742||Microsatellite Marker BAT-26|
|0051743||Microsatellite Marker MONO-27|
|0051745||Microsatellite Marker NR-21|
|0051746||Microsatellite Marker NR-24|
|2001370||Microsatellite Instability Specimen||31208-2|
- BAT -25, BAT-26, MON-27, NR-21, NR-24 genotypes
- HNPCC MSI
- HNPCC tumor screening
- Lynch Syndrome MSI
- Lynch syndrome tumor screening
- microsatellite instability
- MSI Lynch syndrome