Ordering Recommendation

Do not use to screen for celiac disease.  May be used to estimate severity of intestinal villous atrophy in confirmed celiac disease.

Mnemonic
ACTIN IGA
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Tue

Reported

1-8 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma. Contaminated, severely hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
20.0 Units or less Negative
20.1 - 24.9 Units Equivocal
25.0 Units or greater Positive

Interpretive Data

The presence of IgA antibodies against F-Actin in patients having positive serologic markers for celiac disease, such as antiendomysial, anti-transglutaminase, and/or anti-gliadin peptide IgA, indicates the presence of intestinal villus atrophy. Positive results should be confirmed with biopsy.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

83516

Components
Component Test Code* Component Chart Name LOINC
0051725 F-Actin Antibody, IgA 63210-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ACTA
  • Actin Antibody
  • Anti-Smooth Muscle Antibody
  • ASMA
  • F-Actin Antibody
  • SMA
F-Actin (Smooth Muscle) Antibody, IgA