Ordering Recommendation

Primarily used for research and to support attempts to understand the pathogenesis of immune, infectious, allergic, or inflammatory disorders.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube, or plain red.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.4 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Ship in an ARUP Standard Transport Tube.

Unacceptable Conditions

Refrigerated specimens. Contaminated or heat-inactivated specimens.


After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 1 year


Quantitative Multiplex Bead Assay




1-4 days

Reference Interval

Test Number
Reference Interval (pg/mL)
  Interleukin 1 beta, Serum 6.7 or less
  Interleukin 6, Serum 2.0 or less
  Interleukin 8, Serum 3.0 or less
  Tumor Necrosis Factor - alpha, Serum 7.2 or less

Interpretive Data

Results are used to understand the pathophysiology of immune, infectious, or inflammatory disorders, or may be used for research purposes.

Compliance Category

Laboratory Developed Test (LDT)


Cytokine levels may demonstrate diurnal variation. For longitudinal comparison, it is recommended that cytokine levels be determined at the same time of day.

Hotline History


CPT Codes

83520 x3; 83529


Component Test Code* Component Chart Name LOINC
0051536 Interleukin 1 beta, Serum 13629-1
0051537 Interleukin 6, Serum 26881-3
0051538 Interleukin 8, Serum 33211-4
0051539 Tumor Necrosis Factor - alpha, Serum 3074-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • cytokine
  • cytokines
  • IL-1 Beta
  • IL-6
  • IL-8
  • TNF Alpha
Cytokine Panel, Monokines, Serum