Ordering Recommendation

Quantify Epstein-Barr virus (EBV) viral load as an aid in monitoring EBV-related disease.




Quantitative Polymerase Chain Reaction




1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA), Pink (K2EDTA), or Serum Separator Tube (SST). Also acceptable: CSF.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma, whole blood, or CSF to a sterile container. (Min: 0.5 mL)

Storage/Transport Temperature

Whole Blood: Refrigerated.

Unacceptable Conditions

Heparinized specimens.


Specimen source required.


Ambient: 24 hours; Refrigerated: 5 days; Frozen: 1 year
Whole Blood: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 week

Reference Interval

Not detected

Interpretive Data

The quantitative range of this assay is 2.6-7.6 log copies/mL (390-39,000,000 copies/mL)

A negative result (less than 2.6 log copies/mL or less than 390 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen, or EBV DNA nucleic acid in concentrations below the level of detection of the assay.  Inhibition may also lead to underestimation of viral quantitation.

Caution should be taken when interpreting results generated by different assay methodologies.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Analyte Specific Reagent (ASR)


The limit of quantification for this DNA assay is 2.6 log copies/mL (390 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<2.6 log copies/mL (<390 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0051338 EBV by Quantitative PCR, Source 31208-2
0051351 EBV by Quantitative PCR, Log copy/mL 53774-6
0051821 EBV by Quantitative PCR, Interp 5005-4
2002318 EBV by Quantitative PCR, Copy/mL 32585-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • EBV
  • EBV Quant PCR
  • EBV Quant PCR Whole Blood
  • EBV quantitative
  • herpes
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  • Human herpesvirus 4
  • Viral load monitoring
Epstein-Barr Virus by Quantitative PCR (Change effective as of 02/21/23: Refer to 3006079 in the February Hotline)