Ordering Recommendation

Aid in the diagnosis of non-acute (chronic phase) Chagas disease (T. cruzi).

Mnemonic
CHAGAS G
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Wed

Reported

1-8 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated. Also acceptable: Room temperature or frozen.

Unacceptable Conditions

Plasma. Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval

Effective November 9, 2017

1.0 IV or less Negative - No significant level of Trypanosoma cruzi IgG antibody detected.
1.1 IV Equivocal - Questionable presence of Trypanosoma cruzi IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1.2 IV or greater Positive - IgG antibodies to Trypanosoma cruzi detected, which may suggest current or past infection.

Interpretive Data

This assay should not be used for blood donor screening or associated re-entry protocols, or for screening Human Cell and Cellular Tissue-Based Products (HCT/Ps). According to the CDC, at least two different serologic tests should be used to make the laboratory diagnosis of chronic Chagas Disease, as no single serologic test is sufficiently sensitive and specific.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

86753

Components
Component Test Code* Component Chart Name LOINC
0051076 Trypanosoma cruzi Antibody, IgG 59387-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Chagas Antibody, IgG
  • Tcruzi IgG Ab
  • Trypanosoma cruzi Antibody, Total
Trypanosoma cruzi Antibody, IgG