Ordering Recommendation

Not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). Use to detect platelet-specific antibodies in suspected fetal or neonatal alloimmune thrombocytopenia, posttransfusion purpura, or multiplatelet transfusion refractoriness.

Mnemonic
PLT ABSCRN
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Mon-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

For newborns less than 30 days old, collect specimen from the mother.

Collect

Serum separator tube (SST).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube and freeze. (Min: 0.5 mL)

Storage/Transport Temperature

Frozen. Also acceptable: Refrigerated if maintained at temperature for 48 hours or less.

Unacceptable Conditions

Microbially contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 month

Reference Interval

None detected.

Interpretive Data

This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). This test will detect both allo and autoantibodies, but will not distinguish between them. Results of this test should be used in conjunction with clinical findings and other serological tests. Antibodies directed to antigens found on platelets are associated with many different clinical situations. Immune thrombocytopenic purpura (ITP) is a destructive thrombocytopenia caused by autoantibodies. Neonatal alloimmune thrombocytopenia (NATP) and post-transfusion purpura (PTP) are diseases where thrombocytopenia is caused by platelet-specific alloantibodies. HLA alloantibodies do not cause thrombocytopenia, but are commonly associated with refractoriness to platelet transfusions.

This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [PlA1 and PlA2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA Class I antigens (HLA-A-B).

Testing for neonatal alloimmune thrombocytopenia should be performed using a maternal serum, since platelet antibody may not be detected in a neonatal serum. False negative results are common in infant samples.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

86022

Components
Component Test Code* Component Chart Name LOINC
0051062 Platelet Antibodies, Indirect 24375-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Antiplatelet Antibodies
  • Antiplatelet, Circulating Platelet Antibodies
  • Circulating Platelet Antibody
  • HPA-la
  • Indirect Platelet Antibody
  • PLA-1
  • Platelet Antibodies, Qualitative
  • Platelet Antibody, Serum
Platelet Antibodies, Indirect