Diagnose infection from Ehrlichia chaffeensis.
Semi-Quantitative Indirect Fluorescent Antibody
Serum Separator Tube.
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.
Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|0051004||Ehrlichia chaffeensis Antibody, IgG by IFA||< 1:64 Negative-No significant level of Ehrlichia chaffeensis IgG antibody detected.
1:64-1:128 Equivocal-Questionable presence of Ehrlichia chaffeensis IgG antibody detected. Repeat testing in 10-14 days may be helpful.
≥1:256 Positive-Presence of IgG antibody to Ehrlichia chaffeensis detected, suggestive of current or past infection.
|0051003||Ehrlichia chaffeensis Antibody, IgM by IFA||< 1:16 Negative-No significant level of Ehrlichia chaffeensis IgM antibody detected.
≥ 1:16 Positive-Presence of IgM antibody to Ehrlichia chaffeensis detected, suggestive of current or recent infection.
Refer to individual components.
Laboratory Developed Test (LDT)
Human ehrlichiosis is a tick-borne disease caused by rickettsial-like agents. Two forms, human monocytic ehrlichiosis (HME) and human granulocytic ehrlichiosis (HGE), have been described. HME is often referred to as "spotless" or rashless Rocky Mountain spotted fever, and has been reported in various regions of the United States. The causative agent of HME has been identified as Ehrlichia chaffeensis. Infected individuals produce specific antibodies to Ehrlichia chaffeensis which can be detected by an immunofluorescent antibody (IFA) test.
|Component Test Code*||Component Chart Name||LOINC|
|0051003||Ehrlichia chaffeensis Antibody, IgM||48850-2|
|0051004||Ehrlichia chaffeensis Antibody, IgG||47405-6|
- E. chaffeensis antibodies
- E. Chaffeensis IgG, IgM Ab
- Ehrlichiosis IFA test