Recommended test for syphilis screening (reverse algorithm).
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
New York DOH Approval Status
Serum Separator Tube (SST).
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Effective February 16, 2016
0.9 IV or less: Negative - No significant level of Treponema pallidum IgG antibody detected.
1.0 IV: Equivocal - Questionable presence of Treponema pallidum IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1.1 IV or greater: Positive - Presence of IgG antibody to Treponema pallidum detected, suggestive of current or past infection.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time. The Rapid Plasma Reagin (RPR) with Reflex to RPR Titer or T. pallidum Antibody by Particle Agglutination (2007443) is recommended for follow-up of positive results. For CSF specimens, the Treponema pallidum (VDRL) Cerebrospinal Fluid with Reflex to Titer (0050206) test is recommended.
|Component Test Code*||Component Chart Name||LOINC|
|0050920||Treponema pallidum Ab, IgG by ELISA||51838-1|
- Syphilis Antibody, IgG by ELISA
- T pallidum IgG ELISA