Ordering Recommendation

Use for differential evaluation of patients with systemic lupus erythematosus (SLE), Sjögren syndrome, polymyositis/dermatomyositis, and/or other tissue diseases or overlapping syndromes.

Mnemonic
SSA/SSB
Methodology

Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids.  Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Effective May 18, 2015

Test Number
Components
Reference Interval
2012074 SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050692 SSB (La) (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

Interpretive Data

Refer to report.

No compliance statements are in use for this test.

Note
Hotline History
N/A
CPT Codes

86235 x3

Components
Component Test Code* Component Chart Name LOINC
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0050692 SSB (La) (ENA) Antibody, IgG 29965-1
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-ENA Anti-Ribonuclear Protein
  • Ro Ab
  • Scl 70 Antibodies
  • Smith (ENA) Antibody, IgG
  • SSA (Ro) (ENA) Antibody, IgG
  • SSA Ro
  • SSB (La) (ENA) Antibody, IgG
  • SSB LA
Extractable Nuclear Antigen Antibodies (SSA 52, SSA 60, and SSB)