0050652
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  Extractable Nuclear Antigen Antibodies (Smith/RNP, Smith, SSA 52, SSA 60, and SSB)

ENA ABS4
Example Reports
Negative
Positive

Ordering Recommendation

Recommended for the differential diagnosis of systemic lupus erythematosus (SLE) and Sjogren syndrome.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP standard transport tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids. Bacterially contaminated or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Methodology

Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-3 days

Reference Interval

Test Number
 Components
 Reference Interval
  Smith/RNP (ENA) Ab, IgG 40 AU/mL or less
  Smith (ENA) Antibody, IgG 40 AU/mL or less
  SSA-52 (Ro52) (ENA) Antibody, IgG 40 AU/mL or less
  SSA-60 (Ro60) (ENA) Antibody, IgG 40 AU/mL or less
  SSB (La) (ENA) Antibody, IgG 40 AU/mL or less

Interpretive Data




Component
 Interpretation
Smith/RNP (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Smith (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSB (La) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Compliance Category

FDA

Note

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
03/02/2026
X
X
X
X
01/20/2026
X
N/A

CPT Codes

86235 x5

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Components

Component Test Code* Component Chart Name LOINC
0050085 Smith (ENA) Antibody, IgG 33564-6
0050470 Smith/RNP (ENA) Ab, IgG 33567-9
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 70257-1
0050692 SSB (La) (ENA) Antibody, IgG 29965-1
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 107236-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Antibodies to Extractable Nuclear Antigens
  • Ro Ab (ENA)
  • Ro52
  • Ro60 (ENA)
  • Sm Smith Autoantibodies
Extractable Nuclear Antigen Antibodies (Smith/RNP, Smith, SSA 52, SSA 60, and SSB)