Extractable Nuclear Antigen Antibodies (Smith/RNP, Smith, SSA 52, SSA 60, and SSB)

0050652
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Example Reports
Negative
Positive

Ordering Recommendation

Recommended for the differential diagnosis of systemic lupus erythematosus (SLE) and Sjogren syndrome.

Mnemonic

ENA ABS4

Methodology

Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated..

Unacceptable Conditions

Plasma or other body fluids. Bacterially contaminated or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
 Components
 Reference Interval
  Smith/RNP (ENA) Ab, IgG 19 Units or less
  Smith (ENA) Antibody, IgG 40 AU/mL or less
  SSA-52 (Ro52) (ENA) Antibody, IgG 40 AU/mL or less
  SSA-60 (Ro60) (ENA) Antibody, IgG 40 AU/mL or less
  SSB (La) (ENA) Antibody, IgG 40 AU/mL or less

Interpretive Data




Component Interpretation
Smith/RNP (ENA) Antibody, IgG 19 Units or less            Negative
20-39 Units                   Weak Positive
40-80 Units                   Moderate Positive
81 Units or greater      Strong Positive
Smith (ENA) Antibody, IgG 29 AU/mL or less          Negative
30-40 AU/mL                 Equivocal
41 AU/mL or greater    Positive
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less          Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less          Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive
SSB (La) (ENA) Antibody, IgG 29 AU/mL or less            Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive

Compliance Category

FDA

Note

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
02/21/2023
X
02/22/2022
X
09/07/2021
X
X
X
N/A

CPT Codes

86235 x5

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Components

Component Test Code* Component Chart Name LOINC
0050085 Smith (ENA) Antibody, IgG 18323-6
0050470 Smith/RNP (ENA) Ab, IgG 33983-8
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0050692 SSB (La) (ENA) Antibody, IgG 29965-1
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Antibodies to Extractable Nuclear Antigens
  • Ro Ab (ENA)
  • Ro52
  • Ro60 (ENA)
  • Sm Smith Autoantibodies
  • Sm/RNP (ENA)
  • Sm/RNP (ENA) Antibody, IgG
  • Smith (ENA) Antibody, IgG
  • SmRNP Autoantibodies
  • SSA (Ro) (ENA) Antibody, IgG
  • SSA Ro
  • SSA-52
  • SSA-60
  • SSB (La) (ENA) Antibody, IgG
  • Topo-1
  • topoisomerase 1
Extractable Nuclear Antigen Antibodies (Smith/RNP, Smith, SSA 52, SSA 60, and SSB)