Use to evaluate pernicious anemia or immune-mediated deficiency of vitamin B12 with or without megaloblastic anemia. Negative results do not rule out pernicious anemia.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL)
Urine or plasma. Contaminated, heat-inactivated, grossly hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|25.0 Units or greater||Positive|
In the context of vitamin B12 deficiency, the presence of gastric parietal cell antibodies (PCA) and/or intrinsic factor antibodies in association with macrocytic anemia is considered diagnostic for pernicious anemia (PA). However, the presence of gastric PCAs alone is not specific for PA. Gastric PCAs may occur with increased frequency in unaffected family members, a small percentage of healthy individuals, and patients with other autoimmune diseases, such as autoimmune thyroiditis.
Most patients with pernicious anemia have parietal cell antibodies. However, the fact that such antibodies are found with increased frequency in unaffected family members, as well as in patients with other autoimmune diseases, suggests these antibodies do not cause disease by themselves.
|Component Test Code*||Component Chart Name||LOINC|
|0050596||Gastric Parietal Cell Antibody, IgG||40960-7|
- Anti-GPA (AGPA)
- Anti-Parietal Cell Antibody (APCA)
- Hydrogen Potassium (H+/K+) ATPase
- Parietal Cell Ab, IgG
- PCA IgG