Not recommended for herpes simplex virus (HSV) testing; IgM lacks adequate predictive value for acute infection. Molecular testing is preferred (refer to Herpes Simplex Virus by PCR (0060041)).
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Transfer 0.5 mL CSF to an ARUP Standard Transport Tube. (Min: 0.2 mL)
Specimen types other than CSF. Contaminated, heat-inactivated, or hemolyzed specimens.
Indicate source on test request form.
Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year
|0.89 IV or less||Negative - No significant level of detectable HSV IgM antibody.|
|0.90-1.09 IV||Equivocal - Questionable presence of IgM antibodies. Repeat testing in 10-14 days may be helpful.|
|1.10 IV or greater||Positive - IgM antibody to HSV detected, which may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.|
The detection of antibodies to herpes simplex virus in CSF may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer or serum antibodies across the blood-brain barrier.
Fourfold or greater rise in CSF antibodies to herpes on specimens at least 4 weeks apart are found in 74-94% of patients with herpes encephalitis. Specificity of the test based on a single CSF testing is not established. Presently PCR is the primary means of establishing a diagnosis of herpes encephalitis.
|Component Test Code*||Component Chart Name||LOINC|
|0050408||HSV 1 and/or 2 Antibodies IgM, CSF||63432-9|
- HSV antibodies
- HSV IgM