Ordering Recommendation

May aid in the diagnosis of Mycoplasma pneumoniae in patient with persistent pneumonia that is outside of the expected acute phase.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Enzyme-Linked Immunosorbent Assay




1-3 days

Reference Interval

Test Number
Reference Interval
  Mycoplasma Pneumoniae Antibody IgG Less than or equal to 0.09 U/L
  Mycoplasma Pneumoniae Antibody IgM Less than or equal to 0.76 U/L

Interpretive Data

Component Interpretation
Mycoplasma pneumoniae Antibody, IgG < 0.10 U/L: Negative
0.10-0.32 U/L: Equivocal
> 0.32 U/L: Positive
Mycoplasma pneumoniae Antibody, IgM 0.76 U/L or less: Negative - No clinically significant amount of M. pneumoniae IgM antibody detected.
0.77-0.95 U/L: Low Positive - M. pneumoniae-specific IgM presumptively detected.  Collection of a follow-up sample in one to two weeks is recommended to assure reactivity.
0.96 U/L or greater: Positive - Highly significant amount of M. pneumoniae-specific IgM antibody detected.  However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Compliance Category



Hotline History


CPT Codes

86738 x2


Component Test Code* Component Chart Name LOINC
0050397 Mycoplasma Pneumoniae Antibody IgG 5255-5
0050398 Mycoplasma Pneumoniae Antibody IgM 5256-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • M pneumoniae antibodies IgG, IgM
  • M. pneumoniae antibodies
Mycoplasma pneumoniae Antibodies, IgG & IgM