Ordering Recommendation

May aid in the diagnosis of Mycoplasma pneumoniae in patient with persistent pneumonia that is outside of the expected acute phase.


Semi-Quantitative Enzyme-Linked Immunosorbent Assay




1-3 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
Reference Interval
  Mycoplasma Pneumoniae Antibody IgG Less than or equal to 0.09 U/L
  Mycoplasma Pneumoniae Antibody IgM Less than or equal to 0.76 U/L

Interpretive Data

Component Interpretation
Mycoplasma pneumoniae Antibody, IgG < 0.10 U/L: Negative
0.10-0.32 U/L: Equivocal
> 0.32 U/L: Positive
Mycoplasma pneumoniae Antibody, IgM 0.76 U/L or less: Negative - No clinically significant amount of M. pneumoniae IgM antibody detected.
0.77-0.95 U/L: Low Positive - M. pneumoniae-specific IgM presumptively detected.  Collection of a follow-up sample in one to two weeks is recommended to assure reactivity.
0.96 U/L or greater: Positive - Highly significant amount of M. pneumoniae-specific IgM antibody detected.  However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Compliance Category



Hotline History


CPT Codes

86738 x2


Component Test Code* Component Chart Name LOINC
0050397 Mycoplasma Pneumoniae Antibody IgG 5255-5
0050398 Mycoplasma Pneumoniae Antibody IgM 5256-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • M pneumoniae antibodies IgG, IgM
  • M. pneumoniae antibodies
Mycoplasma pneumoniae Antibodies, IgG & IgM