Provide retrospective evidence of suspected Legionella pneumophila infection.
Semi-Quantitative Indirect Fluorescent Antibody
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.
Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
<1:128 Negative - No significant level of Legionella pneumophila Type 1-6 IgG antibody detected.
1:128 Equivocal - Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1:256 or greater Positive - Presence of Legionella pneumophila Type 1-6 IgG antibody detected, suggestive of current or past infection.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. This assay may detect infection by any of the serotypes 1-6. The CDC and many state health laboratories recommend testing only for antibody to Legionella pneumophila Type 1. For equivocal or positive IFA results, the CDC protocol suggests follow-up testing for Legionella pneumophila antibody Type 1.
|Component Test Code*||Component Chart Name||LOINC|
|0050365||Legionella Pneumophila 1-6,IgG||53770-4|
- L pneumophila IgG
- L pneumophila Types 1-6 Ab
- Legionella pneumophila IgG antibody
- Legionnaires Disease Testing