Ordering Recommendation

May be useful when acute legionellosis is suspected; however, Legionella pneumophila Antigen, Urine (0070322) is preferred.

Mnemonic
LEGAB1-6M
Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Performed

Mon-Fri

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cell ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

< 1:16

Interpretive Data

IgM antibody to Legionella pneumophila serotypes 1-6 is measured using an IgM-specific conjugate.  It is recommended that the IgM test always be performed in conjunction with IgG antibody test.

The IgM response to Legionella tends to develop concurrently with the IgG response and may remain elevated as long as the IgG response remains elevated.  Cross-reactions have been described with several species of bacteria and Mycoplasma.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

86713

Components
Component Test Code* Component Chart Name LOINC
0050274 Legionella Pneumophila 1-6,IgM 16133-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • L pneumophila IgM
  • L pneumophila Types 1-6 Ab
  • Legionella pneumophila IgM antibody
  • Legionnaires Disease Testing
Legionella pneumophila Antibody (Types 1-6), IgM by IFA