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Borrelia burgdorferi Antibodies, IgG and IgM by Immunoblot
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Ordering Recommendation
Do not order in the absence of a positive/equivocal ELISA screening test. IgM immunoblot is not useful after the first 4 weeks of clinical symptoms.
Mnemonic
Methodology
Qualitative Immunoblot
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
Specimen Required
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
Refrigerated.
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
Effective August 15, 2011
Negative
Interpretive Data
IgG: For this assay, a positive result is reported when any 5 or more of the following 10 bands are present: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. All other banding patterns are reported as negative.
IgM: For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.
FDA
Note
Per CDC guidelines, if ELISA test result is NEGATIVE, immunoblot should not be performed.
This test should be used for confirmation of an equivocal or positive B. burgdorferi total antibodies, IgG and/or IgM test performed on patients less than 4 weeks after appearance of erythema migrans.
Hotline History
CPT Codes
86617 x2
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0050252 | B. burgdorferi IgG Immunoblot | 6320-6 |
| 0050253 | B. burgdorferi Antibody IgM Immunoblot | 6321-4 |
Aliases
- Lyme Antibodies
- Lyme Disease
- Lyme Disease Ab Western Blot
- Lyme Disease Antibodies (Serum), WB
- Lyme Disease antibodies WB
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