Do not order in the absence of a positive/equivocal ELISA screening test. IgM immunoblot is not useful after the first 4 weeks of clinical symptoms.
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Effective August 15, 2011
IgG: For this assay, a positive result is reported when any 5 or more of the following 10 bands are present: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. All other banding patterns are reported as negative.
IgM: For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.
Per CDC guidelines, if ELISA test result is NEGATIVE, immunoblot should not be performed.
This test should be used for confirmation of an equivocal or positive B. burgdorferi total antibodies, IgG and/or IgM test performed on patients less than 4 weeks after appearance of erythema migrans.
|Component Test Code*||Component Chart Name||LOINC|
|0050252||B. burgdorferi IgG Immunoblot||6320-6|
|0050253||B. burgdorferi Antibody IgM Immunoblot||6321-4|
- Lyme Antibodies
- Lyme Disease
- Lyme Disease Ab Western Blot
- Lyme Disease Antibodies (Serum), WB
- Lyme Disease antibodies WB