Ordering Recommendation

Detect presence of IgG and IgM antibodies in individuals with a clinical suspicion of West Nile Virus.

Mnemonic
WNILE SER
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Sun, Tue, Fri

Reported

1-6 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
0050234 West Nile Virus Antibody, IgG by ELISA, Serum 1.29 IV or less: Negative - No significant level of West Nile virus IgG antibody detected.
1.30-1.49 IV: Equivocal - Questionable presence of West Nile virus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1.50 IV or greater: Positive - Presence of IgG antibody to West Nile virus detected, suggestive of current or past infection. 
0050236 West Nile Virus Antibody, IgM by ELISA, Serum 0.89 IV or less: Negative - No significant level of West Nile virus IgM antibody detected.
0.90-1.10 IV: Equivocal - Questionable presence of West Nile virus IgM antibody detected. Repeat testing in 10-14 days may be helpful.
1.11 IV or greater: Positive - Presence of IgM antibody to West Nile virus detected, suggestive of current or recent infection. 

Interpretive Data

Refer to individual components for additional interpretive data.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

86789; 86788

Components
Component Test Code* Component Chart Name LOINC
0050234 West Nile Virus Ab, IgG, Ser 38997-3
0050236 West Nile Virus Ab, IgM, Ser 38166-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Arbovirus
  • Flavivirus
  • West Nile Virus Antibodies
  • WNV
  • WNV IgG and IgM
West Nile Virus Antibodies, IgG and IgM by ELISA, Serum