Ordering Recommendation

May aid in the diagnosis of acute Epstein-Barr virus (EBV) infection and EBV reactivation in conjunction with other serologic studies. Molecular tests are preferred to detect EBV reactivation. Not a stand-alone test.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.

Storage/Transport Temperature


Unacceptable Conditions

Contaminated,  heat-inactivated or grossly hemolyzed specimens.


Label specimens plainly as "acute" or "convalescent."


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Chemiluminescent Immunoassay




1-2 days

Reference Interval

Effective February 19, 2013

8.9 U/mL or less: Not Detected
9.0-10.9 U/mL: Indeterminate - Repeat testing in 10-14 days may be helpful.
11.0 U/mL or greater: Detected

Interpretive Data

Compliance Category



EBV EA-D values obtained with different manufacturers' assay methods may not be used interchangeably.  The magnitude of the reported EBV EA-D level cannot be correlated to an endpoint titer.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0050225 EBV Antibody to Early (D) Antigen IgG 50969-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • EA-D IgG Ab
  • EAD Ab
  • EBNA Antibody
  • EBNA to EA-D
  • EBV Antibody to Early D Antigen
  • EBV Nuclear Antibody
Epstein-Barr Virus Antibody to Early D Antigen (EA-D), IgG