Ordering Recommendation

Secondary screening for systemic lupus erythematosus (SLE) based on ANA results.

Mnemonic
DNA
Methodology

Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Effective August 20, 2012

Test Number
Components
Reference Interval
Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA None Detected.
2002693 Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10

Interpretive Data

Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent test (ELISA). All ELISA results reported as "detected" (positive) are confirmed by a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA. Additional information and recommendations for testing may be found at https://www.arupconsult.com/Topics/AutoimmuneDz/ConnectiveTissueDz/index.html.

No compliance statements are in use for this test.

Note

If dsDNA IgG antibody is detected, the result will be confirmed by Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae). Additional charges apply.

Hotline History
N/A
CPT Codes

86225; if reflexed, add 86256

Components
Component Test Code* Component Chart Name LOINC
0050215 Double-Stranded DNA (dsDNA) Ab IgG ELISA 58465-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • anti-DNA
  • Anti-dsDNA Antibody
  • Anti-Native DNA
  • Antibody to ds-DNA
  • Crithidia luciliae
  • DNA ds Ab
  • Double stranded DNA antibody
  • ds DNA Ab
  • ds-DNA
  • ds-DNA IgG
  • dsDNA reflex
  • dsDNA reflex panel
  • Native double-stranded DNA antibody
Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA