Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA
Secondary screening for systemic lupus erythematosus (SLE) based on ANA results.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
New York DOH Approval Status
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Plasma. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)
||Reference Interval (IUs)
|Double-Stranded DNA (dsDNA) Ab IgG ELISA||24 IUs or less|
Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent assay (ELISA). If ordered as reflex (0050215), positive ELISA results (>24 IU) will be reflexed to a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]) for confirmation. Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA. Additional information and recommendations for testing may be found at https://arupconsult.com/content/systemic-lupus-erythematosus.
|dsDNA (Double Stranded DNA) Antibody, IgG||24 IUs or less : Negative
25 to 30 IUs : Borderline Positive
30 to 60 IUs : Low Positive
60 to 200 IUs : Positive
201 IUs or greater : Strong Positive
If dsDNA IgG antibody is detected, the result will be confirmed by Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae). Additional charges apply.
86225; if reflexed, add 86256
|Component Test Code*||Component Chart Name||LOINC|
|0050215||Double-Stranded DNA (dsDNA) Ab IgG ELISA||58465-6|
- Anti-dsDNA Antibody
- Anti-Native DNA
- Antibody to ds-DNA
- Crithidia luciliae
- DNA ds Ab
- Double stranded DNA antibody
- ds DNA Ab
- ds-DNA IgG
- dsDNA reflex
- dsDNA reflex panel
- Native double-stranded DNA antibody