Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA

0050215
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Example Reports
Detected
None Detected

Ordering Recommendation

Secondary screening for systemic lupus erythematosus (SLE) based on ANA results.

Mnemonic

DNA

Methodology

Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Effective May 17, 2021

Test Number
 Components
 Reference Interval
  Double-Stranded DNA (dsDNA) Ab IgG ELISA
24 IUs or less
25 to 30 IUs
30 to 60 IUs
60 to 200 IUs
201 IUs or greater		 Negative
Borderline Positive
Low Positive
Positive
Strong Positive

2002693 Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10

Interpretive Data

Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent test (ELISA). All ELISA results reported as "detected" (positive) are confirmed by a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA. Additional information and recommendations for testing may be found at https://www.arupconsult.com/Topics/AutoimmuneDz/ConnectiveTissueDz/index.html.

Compliance Category

FDA

Note

If dsDNA IgG antibody is detected, the result will be confirmed by Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae). Additional charges apply.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
05/17/2021
X
X
N/A

CPT Codes

86225; if reflexed, add 86256

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Components

Component Test Code* Component Chart Name LOINC
0050215 Double-Stranded DNA (dsDNA) Ab IgG ELISA 58465-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • anti-DNA
  • Anti-dsDNA Antibody
  • Anti-Native DNA
  • Antibody to ds-DNA
  • Crithidia luciliae
  • DNA ds Ab
  • Double stranded DNA antibody
  • ds DNA Ab
  • ds-DNA
  • ds-DNA IgG
  • dsDNA reflex
  • dsDNA reflex panel
  • Native double-stranded DNA antibody
Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA