Recommended for the evaluation of systemic lupus erythematosus (SLE).
Quantitative Immunoturbidimetry/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody/Quantitative Chemiluminescent Immunoassay/Semi-Quantitative Multiplex Bead Assay
Serum separator tube.
Allow specimen to clot for 30 to 60 minutes at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 4 mL serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 2 mL)
Plasma. Specimens left to clot while refrigerated. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 1 hour; Refrigerated: 24 hours, Frozen: 2 weeks
Initial testing includes RF, C3, C4, and ANA. Specimens are screened for ANA using ELISA. If antibodies are detected, then an IFA titer will be added. ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge. If confirmed by IFA, then specimen will be tested for Thyroid Peroxidase (TPO) Antibody; Anti-Scl-70 (ENA), EIA; Smith/RNP (ENA) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 and 60 (Ro) (ENA) Antibodies, IgG; SSB (La) (ENA) Antibody, IgG; and Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; if Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA result is Detected, then Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) is added. Additional charges apply.
86431; 86160 x2; 86038; if reflexed, add 86039; if reflexed, add 86235 x6; 86376; 86225; if reflexed, add 86256
|Component Test Code*||Component Chart Name||LOINC|
|0050080||Anti-Nuclear Ab (ANA), IgG by ELISA||29950-3|
|0050150||Complement Component 3||4485-9|
|0050155||Complement Component 4||4498-2|
- ANA IgG
- Anti-Nuclear Ab (ANA), IgG
- Complement C3
- Complement C4
- Complement Component 3
- Complement Component 4
- Lupus Panel
- Lupus Reflex Panel
- RA factor
- Rheumatoid Factor