Ordering Recommendation

Recommended for the evaluation of systemic lupus erythematosus (SLE).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Allow specimen to clot for 30 to 60 minutes at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 4 mL serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 2 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma. Specimens left to clot while refrigerated. Contaminated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 8 hours; Refrigerated: 1 Week, Frozen: 2 weeks


Quantitative Immunoturbidimetry/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody/Quantitative Chemiluminescent Immunoassay/Semi-Quantitative Multiplex Bead Assay




1-5 days

Reference Interval

By report

Interpretive Data

Compliance Category



Initial testing includes RF, C3, C4, and ANA. Specimens are screened for ANA using ELISA. If antibodies are detected, then an IFA titer will be added. ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge. If confirmed by IFA, then specimen will be tested for Thyroid Peroxidase (TPO) Antibody; Anti-Scl-70 (ENA); Smith/RNP (ENA) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 and 60 (Ro) (ENA) Antibodies, IgG; SSB (La) (ENA) Antibody, IgG; and Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; if Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA result is Detected, then Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) is added. Additional charges apply.

Hotline History


CPT Codes

86431; 86160 x2; 86038; if reflexed, add 86039; if reflexed, add 86235 x6; 86376; 86225; if reflexed, add 86256


Component Test Code* Component Chart Name LOINC
0050080 Anti-Nuclear Ab (ANA), IgG by ELISA 29950-3
0050150 Complement Component 3 4485-9
0050155 Complement Component 4 4498-2
0050465 Rheumatoid Factor 11572-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • ANA IgG
  • Anti-Nuclear Ab (ANA), IgG
  • Complement C3
  • Complement C4
  • Complement Component 3
  • Complement Component 4
  • Lupus Panel
  • Lupus Reflex Panel
  • RA factor
  • RF
  • Rheumatoid Factor
Lupus Comprehensive Reflexive Panel