Ordering Recommendation

Aid in the detection of amebic liver abscess. Test is not useful for intestinal infection.

Mnemonic
AMB
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Tue, Fri

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Effective February 6, 2017

8 U or less: Negative - No significant level of detectable E. histolytica IgG antibody.
9 - 11 U: Equivocal - Repeat testing in 10-14 days may be helpful.
12 U or greater: Positive - IgG antibody to E. histolytica detected, suggestive of a current or past infection.

Interpretive Data

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.

Compliance Category

FDA

Note

In the case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative.

Hotline History
N/A
CPT Codes

86753

Components
Component Test Code* Component Chart Name LOINC
0050070 E. histolytica (amebiasis), Ab, IgG 9522-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Amebiasis histolytica Antibody
  • E histolytica IgG Antibody
  • E. histolytica Antibody
  • Ova and Parasite Exam
Entamoeba histolytica (amebiasis), Antibody, IgG