Ordering Recommendation

Aid in the detection of amebic liver abscess. Test is not useful for intestinal infection.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Enzyme-Linked Immunosorbent Assay


Tue, Fri


1-5 days

Reference Interval

Effective February 6, 2017

8 U or less: Negative - No significant level of detectable E. histolytica IgG antibody.
9 - 11 U: Equivocal - Repeat testing in 10-14 days may be helpful.
12 U or greater: Positive - IgG antibody to E. histolytica detected, suggestive of a current or past infection.

Interpretive Data

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.

Compliance Category



In the case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0050070 E. histolytica (amebiasis), Ab, IgG 9522-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Amebiasis histolytica Antibody
  • E histolytica IgG Antibody
  • E. histolytica Antibody
  • Ova and Parasite Exam
Entamoeba histolytica (amebiasis), Antibody, IgG