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Immunohistochemistry Stain Form Recommended (ARUP form #32978)
Ordering Recommendation

Aid in prediction of response to HER2-directed therapy in patients with breast carcinoma or gastroesophageal adenocarcinoma. Alternate test to confirm equivocal dual in situ hybridization (ISH) or fluorescence in situ hybridization (FISH) result. Refer to ARUP Immunohistochemistry Stain Offerings brochure at www.aruplab.com/ap/resources.

Mnemonic
HERCEPIP
Methodology

Immunohistochemistry

Performed

Mon-Fri

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tumor Tissue

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue within 30 minutes of removal from patient. Fixative duration: 6-72 hours. Transport tissue block or 5 unstained (3-to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800)522-2787. (Min: 3 slides) If sending precut slides, do not oven bake.

Storage/Transport Temperature

Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Paraffin block with no tumor tissue remaining. Specimens with fixation delayed for more than 30 minutes. Specimens fixed in any other fixative other than 10 percent neutral buffered formalin. Tissue fixed for less than 6 hours or greater than 72 hours. Cytology specimens fixed in alcohol. Decalcified specimens.

Remarks

Pathology report including tissue source and tumor origin must be submitted. Document time from tissue acquisition to fixation and fixation duration on requisition or enter at time of order. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely ; Frozen: Unacceptable

Reference Interval
Interpretive Data

Refer to report.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

88360

Components
Component Test Code* Component Chart Name LOINC
0049171 Homogenous Pattern
0049184 Intensity of Staining
0049188 Percent of Cells/Circumferential
0049202 ERBB2 (HercepTest) by IHC 18474-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • c-erbB2 Hercept IHC
  • ERBB2 Hercept IHC
  • HER-2/neu Hercept IHC
  • HER2
  • HER2 IHC
  • Herceptin
ERBB2 (HER2/neu) (HercepTest) with Interpretation by Immunohistochemistry, Tissue