Anatomic Pathology Test Request Form Recommended (ARUP form #32960)

Ordering Recommendation

Aid in differential diagnosis of neutrophilia, including chronic myeloid leukemia (CML) and leukemoid reaction.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Green (sodium or lithium heparin).

Specimen Preparation

Protect both smears and whole blood from light and pack accordingly to avoid breakage. Transport 5 mL whole blood (Min: 1 mL) AND 6 unfixed, well-prepared smears (Min: 6 smears)

Storage/Transport Temperature

Room temperature. Send Sunday through Thursday only. Whole blood must be received within 24 hours of collection; smears must be made within 24 hours of collection and received within 7 days of collection.

Unacceptable Conditions

Specimens collected in EDTA. Poorly prepared smears (too thick or no feather edge). Broken or fixed smears.  Specimens that have been spun.  Smears made from anything other than heparin.  Smears made from blood older than 24 hours.  Whole blood not protected from light.  Smears not protected from light.


Further information on how to make an adequate smear can be found in the following instructional video: https://www.youtube.com/watch?v=ca3NwrlpS40&feature=youtube


 Blood: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Unfixed Smears:
Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable


Cytochemical Stain




1-5 days

Reference Interval

Female: 33-149 (no units)
Male: 22-124 (no units)

Interpretive Data

Refer to Report

Compliance Category



Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0049000 Leukocyte Alkaline Phosphatase 4659-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • LAP
Leukocyte Alkaline Phosphatase (Test on Delay as of 7/21/2023)