Aids in treatment decisions in individuals with solid tumors including colorectal and non-small cell lung cancers. Does not cover extended RAS; detects mutations in KRAS codons 12, 13, and 61 only.
Polymerase Chain Reaction/Pyrosequencing
DNA isolation: Sun-Sat
Assay: Varies
7-12 days
Tumor tissue. Also acceptable: DNA extracted by CLIA certified lab with corresponding client-circled H&E slide.
Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Transport block(s) and/or slide(s) in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Extracted DNA: Refrigerated.
Less than 25 percent tumor. DNA extracted by a non-CLIA lab. DNA extracted without a corresponding circled H&E slide. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.
Include surgical pathology report.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
This assay detects mutations in codons 12, 13, and 61.
Hotline History
88381; 81275; 81276
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0040249 | KRAS Mutation Detection | 21702-6 |
2002148 | Block ID | 57723-9 |
- anti-EGFR therapy resistance testing
- Cetuximab (Erbitux)
- Codon 12,13
- Codon 61
- KRAS codons 12, 13, 61
- Panitumumab (Vectibix)
- RAS