Ordering Recommendation

Use to quantitate JAK2 V617F allele frequency from peripheral whole blood. Aids in risk stratification and therapeutic monitoring of JAK2 V617F mutation positive myeloproliferative neoplasms.

Mnemonic

JAK2 QNT

Methodology

Polymerase Chain Reaction

Performed

DNA isolation: Sun-Sat
Assay:
Mon, Wed, Fri

Reported

5-9 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA). Also acceptable: DNA extracted by CLIA certified lab.

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL).
Extracted DNA: Transport 40uL DNA with at least 50 ng/uL concentration (Min: 40uL). Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, bone marrow, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely

Reference Interval

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

81270

Components

Component Test Code* Component Chart Name LOINC
0040167 JAK2 Gene, V617F Mutation, Quantitative 53761-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • BCR-ABL1-negative tesing
  • Classic BCR-ABL1-negative MPN testing
  • MPN JAK 2
  • mutant JAK 2 V617F allelic burden
JAK2 Gene, V617F Mutation, Quantitative (INACTIVE as of 05/17/21: Refer to 3003751 in the May Hotline)