Ordering Recommendation

Detect fetal hemoglobin in maternal blood to determine the presence and extent of fetal-maternal hemorrhage. Determine appropriate dose of Rh immunoglobulin therapy in Rh negative mothers. 

Mnemonic
KB
Methodology

Semi-Quantitative Acid Elution Eosin Stain/Microscopy

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA) or Pink (K2EDTA).

Specimen Preparation

Transport 3 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimens collected after Rh immunoglobulin administered, Cord or Fetal blood, Clotted specimens.

Remarks
Stability

Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data

Volume (mL) of fetomaternal hemorrhage (assuming a maternal blood volume of 5 liters) = percent fetal rbcs x50. 

The quantitation of fetomaternal hemorrhage by this method is inherently imprecise.  Therefore, it is important to provide a safety margin when calculating the dose of RhIG.

Increased Acid Resistant Cells may be found in some hemoglobinopathies, beta thalassemias and hereditary persistence of fetal hemoglobin. Results must be interpreted with caution in these situations.

No compliance statements are in use for this test.

Note

This test should be ordered on pregnant or post-partum women who need to be assessed for RhoGAM® or fetal-maternal bleeds. For routine fetal hemoglobin (Hb F) testing, please order Hemoglobin Evaluation with Reflex to Electrophoresis and/or HPLC (0050610).

Hotline History
N/A
CPT Codes

85460

Components
Component Test Code* Component Chart Name LOINC
0040105 Kleihauer-Betke Stain 4579-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Fetal hemoglobin stain
  • KB test
  • Kleihauer-Betke Stain
Kleihauer-Betke Stain for Fetal Hemoglobin(INACTIVE as of 8/17/2020: Refer to test #2001743, Fetal Hemoglobin Determination for Feto maternal Hemorrhage.)