APC Resistance Profile with Reflex to Factor V Leiden
Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden variant. Refer to Factor V Leiden (F5) R506Q Mutation (0097720) for individuals with prolonged baseline clotting times due to anticoagulation or a lupus anticoagulant.
Electromagnetic Mechanical Clot Detection/Polymerase Chain Reaction/Fluorescence Monitoring
New York DOH Approval Status
Light Blue (Sodium Citrate) AND Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.
Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)
Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Whole Blood: Frozen.
Serum, clotted or hemolyzed specimens. Frozen specimens in glass collection tubes.
Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 1 month
|APC Resistance||2.00 or greater|
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency or presence of direct oral anticoagulants (DOACs) may cause an unreliable ratio.
Note: If APC resistance is normal, then no further testing will be added. If APC resistance is low, or if a valid result cannot be obtained for the APC portion of the profile, then Factor V Leiden by PCR will be added. Additional charges apply.
If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
85307; if reflexed, add 81241
|Component Test Code*||Component Chart Name||LOINC|
|0030194||Factor V Leiden by PCR||21668-9|
|2001308||FACV REF Specimen||31208-2|
- Activated Protein C Resistance
- Factor V Leiden Functional Assay with Reflex to PCR
- Protein C Resistance