0030192
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APC Resistance Profile with Reflex to Factor V Leiden

APC R
Example Reports
Heterozygous
Homozygous

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Informed Consent for Genetic Testing (required for NY patients)

Ordering Recommendation

Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden variant. Refer to Factor V Leiden (F5) R506Q Mutation (0097720) for individuals with prolonged baseline clotting times due to anticoagulation or a lupus anticoagulant.

Methodology

Electromagnetic Mechanical Clot Detection/Polymerase Chain Reaction/Fluorescence Monitoring

Performed

Mon-Sat

Reported

1-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Light Blue (Sodium Citrate) AND Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)

Storage/Transport Temperature

Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Whole Blood: Frozen.

Unacceptable Conditions

Serum, clotted or hemolyzed specimens. Frozen specimens in glass collection tubes.

Remarks
Stability

Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 1 month

Reference Interval

Test Number
 Components
 Reference Interval
  APC Resistance 2.00 or greater

Interpretive Data

Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency or presence of direct oral anticoagulants (DOACs) may cause an unreliable ratio.

Note: If APC resistance is normal, then no further testing will be added. If APC resistance is low, or if a valid result cannot be obtained for the APC portion of the profile, then Factor V Leiden by PCR will be added. Additional charges apply.

Compliance Category

FDA

Note

If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
04/04/2022
X
N/A

CPT Codes

85307; if reflexed, add 81241

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Components

Component Test Code* Component Chart Name LOINC
0030193 APC Resistance 13590-5
0030194 Factor V Leiden by PCR 21668-9
2001308 FACV REF Specimen 31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Activated Protein C Resistance
  • APCR
  • Factor V Leiden Functional Assay with Reflex to PCR
  • Protein C Resistance
APC Resistance Profile with Reflex to Factor V Leiden