Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden variant.
Electromagnetic Mechanical Clot Detection/Polymerase Chain Reaction/Fluorescence Monitoring
Light Blue (Sodium Citrate) AND Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.
Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)
Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Whole Blood: Refrigerated.
Serum, clotted or hemolyzed specimens.
Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.
No compliance statements are in use for this test.
If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
85307; if reflexed, add 81241
|Component Test Code*||Component Chart Name||LOINC|
|0030194||Factor V Leiden by PCR||21668-9|
|2001308||FACV REF Specimen||31208-2|
- Activated Protein C Resistance
- Factor V Leiden Functional Assay with Reflex to PCR
- Protein C Resistance