Ordering Recommendation

Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden variant.




Electromagnetic Mechanical Clot Detection/Polymerase Chain Reaction/Fluorescence Monitoring




1-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Light Blue (Sodium Citrate) AND Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)

Storage/Transport Temperature

Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Whole Blood:

Unacceptable Conditions

Serum, clotted or hemolyzed specimens. Frozen specimens in glass collection tubes.


Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

Test Number
Reference Interval
0030127 APC Resistance Profile 2.0 or greater
0097720 Factor V Leiden (F5) R506Q Mutation Negative: This sample is negative for factor V Leiden, R506Q mutation.

Interpretive Data

Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.

Compliance Category



If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.

Hotline History


CPT Codes

85307; if reflexed, add 81241


Component Test Code* Component Chart Name LOINC
0030193 APC Resistance 13590-5
0030194 Factor V Leiden by PCR 21668-9
2001308 FACV REF Specimen 31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Activated Protein C Resistance
  • APCR
  • Factor V Leiden Functional Assay with Reflex to PCR
  • Protein C Resistance
APC Resistance Profile with Reflex to Factor V Leiden