Ordering Recommendation

Use when total alkaline phosphatase activity is elevated to determine amounts contributed by bone and liver isoenzymes.

Mnemonic
ALKP-ISO
Methodology

Quantitative Heat Inactivation/Enzymatic

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST) or Green (Sodium or Lithium Heparin).

Specimen Preparation

Allow serum specimen to clot completely at room temperature. Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube and refrigerate or freeze immediately. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimens collected in EDTA, sodium fluoride, sodium citrate, or potassium oxalate. Grossly hemolyzed or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 1 week; Refrigerated: 1 week; Frozen: 2 months

Reference Interval
Test Number
Components
0020005 Alkaline Phosphatase, Serum or Plasma Male
0-30 days: 60-320 U/L
1-11 months: 70-350 U/L
1-3 years: 125-320 U/L
4-6 years: 150-370 U/L
7-9 years: 150-440 U/L
10-11 years: 150-470 U/L
12-13 years: 160-500 U/L
14-15 years: 130-530 U/L
16-19 years: 60-270 U/L
20 years and older: 40-120 U/L
Female
0-30 days: 60-320 U/L
1-11 months: 70-350 U/L
1-3 years: 125-320 U/L
4-6 years: 150-370 U/L
7-9 years: 150-440 U/L
10-11 years: 150-530 U/L
12-13 years: 110-525 U/L
14-15 years: 55-305 U/L
16-19 years: 40-120 U/L
20 years and older: 40-120 U/L
Bone Male
1-6 years: 0-208 U/L
7-9 years: 0-264 U/L
10-15 years: 0-340 U/L
16-19 years: 0-165 U/L
20 years and older: 0-55 U/L
Female
1-6 years: 0-189 U/L
7-9 years: 0-246 U/L
10-13 years: 0-340 U/L
14-15 years: 0-91 U/L
16 years and older: 0-55 U/L
Liver Male
1-6 years: 0-145 U/L
7-11 years: 0-182 U/L
12-15 years: 0-226 U/L
16-19 years: 0-114 U/L
20 years and older: 0-94 U/L
Female
1-9 years: 0-148 U/L
10-15 years: 0-162 U/L
16 years and older: 0-94 U/L

Interpretive Data

Bone Specific Alkaline Phosphatase (0070053) and 5'Nucleotidase (0080235) may be useful in identifying disorders of bone and liver, respectively.

Compliance Category

FDA

Note

The presence of heat-stable isoenzymes (i.e., germ cell and placental) will be detected but their activities cannot be differentiated from the liver isoenzyme.

Hotline History
N/A
CPT Codes

84075; 84080

Components
Component Test Code* Component Chart Name LOINC
0020005 Alkaline Phosphatase 6768-6
0021006 Alk-Phosphatase Other Calc 33421-9
0021011 Alk-Phosphatase Bone Calc 1777-2
0021016 Alk-Phosphatase Liver Calc 1779-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ALK PHOS
  • Alkaline Phosphatase
  • Alkaline Phosphatase Isoenzymes by Heat Fractionation
  • Alkp
Alkaline Phosphatase Isoenzymes, Serum or Plasma