Ordering Recommendation

Do not use to detect myocardial injury. Troponin I (0090613) and Troponin T (cTnT) 5th Generation (3001831) are the recommended tests for diagnosis and management of acute coronary syndrome. In rare cases, this test may be used to evaluate elevated lactate dehydrogenase associated with noncardiac muscle injury.

Mnemonic
LDISO
Methodology

Quantitative Enzymatic/Electrophoresis

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST) or Plain Red.

Specimen Preparation

Do not refrigerate or freeze. Allow specimen to clot completely at room temperature. Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Hemolyzed specimens.

Remarks
Stability

After separation of cells: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

LD-1: 14-27%
LD-2: 29-42%
LD-3: 18-30%
LD-4: 8-15%
LD-5: 6-23%

Lactate Dehydrogenase, Total:
0-1 month: 200-465 U/L
2-17 months: 200-450 U/L
18 months-10 years: 165-430 U/L
11-16 years: 127-287 U/L
17 years and older: 105-230 U/L

Interpretive Data



Compliance Category

FDA

Note

LD-1 and LD-2 are elevated in hemolyzed specimens and serum which has not been separated from cells.  LD-3, LD-4, and LD-5 are labile at low temperatures, and are erroneously low in specimens that have been refrigerated or frozen.

Hotline History
N/A
CPT Codes

83625; 83615

Components
Component Test Code* Component Chart Name LOINC
0020015 Lactate Dehydrogenase - 1 2536-1
0020016 Lactate Dehydrogenase - 2 2539-5
0020017 Lactate Dehydrogenase - 3 2542-9
0020018 Lactate Dehydrogenase - 4 2545-2
0020019 Lactate Dehydrogenase - 5 2548-6
0020107 Lactate Dehydrogenase, Total 14804-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Isoenzymes
  • Isoenzymes of Lactate Dehydrogenase
  • Isoenzymes, LD
  • Lactate Dehydrogenase Isoenzymes, S
  • LD
  • LDH
  • LDH Fractionation
  • LDH Isoenzymes
Lactate Dehydrogenase, Isoenzymes