Ordering Recommendation

Do not use to detect myocardial injury. Troponin I (0090613) and Troponin T (cTnT) 5th Generation (3001831) are the recommended tests for diagnosis and management of acute coronary syndrome. In rare cases, this test may be used to evaluate elevated lactate dehydrogenase associated with noncardiac muscle injury.




Quantitative Enzymatic Assay/Electrophoresis




1-2 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST) or Plain Red.

Specimen Preparation

Do not refrigerate or freeze. Allow specimen to clot completely at room temperature. Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Hemolyzed specimens.


After separation of cells: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

LD-1: 14-27%
LD-2: 29-42%
LD-3: 18-30%
LD-4: 8-15%
LD-5: 6-23%

Lactate Dehydrogenase, Total:
0-1 month: 200-465 U/L
2-17 months: 200-450 U/L
18 months-10 years: 165-430 U/L
11-16 years: 127-287 U/L
17 years and older: 105-230 U/L

Interpretive Data

Compliance Category



LD-1 and LD-2 are elevated in hemolyzed specimens and serum which has not been separated from cells.  LD-3, LD-4, and LD-5 are labile at low temperatures, and are erroneously low in specimens that have been refrigerated or frozen.

Hotline History


CPT Codes

83625; 83615


Component Test Code* Component Chart Name LOINC
0020015 Lactate Dehydrogenase - 1 2536-1
0020016 Lactate Dehydrogenase - 2 2539-5
0020017 Lactate Dehydrogenase - 3 2542-9
0020018 Lactate Dehydrogenase - 4 2545-2
0020019 Lactate Dehydrogenase - 5 2548-6
0020107 Lactate Dehydrogenase, Total 14804-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Isoenzymes
  • Isoenzymes of Lactate Dehydrogenase
  • Isoenzymes, LD
  • Lactate Dehydrogenase Isoenzymes, S
  • LD
  • LDH
  • LDH Fractionation
  • LDH Isoenzymes
Lactate Dehydrogenase, Isoenzymes