Ordering Recommendation

Order this assay only when a specimen is repeatedly reactive for HIV-1 or HIV 1-2 antibodies.

Mnemonic
HIV WBLOT
Methodology

Qualitative Western Blot

Performed

Varies

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube. Also acceptable: Lavender (EDTA), green (sodium or lithium heparin) or lt. blue (sodium citrate).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: Up to 1 week is acceptable, but not preferred; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)

Reference Interval

Negative

Interpretive Data

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Compliance Category

FDA

Note

Order this assay only when a specimen is repeatedly reactive for HIV 1 or HIV 1-2 antibodies.

Hotline History
N/A
CPT Codes

86689

Components
Component Test Code* Component Chart Name LOINC
0020284 HIV-1 Antibody Confirm, Western Blot 5221-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HIV 1 Ab
  • HIV 1 Antibody Confirmation
  • HIV 1 western blot
  • HIV confirmation test
  • HIV-1
Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot