Ordering Recommendation

Use to determine the clinical significance of cold reacting autoantibodies. Before ordering the Thermal Amplitude Test, results from the Antibody ID Package (IRL) (0013003) are required to identify specific antibodies that may interfere with testing.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Three 7 ml lavender (K2EDTA) or pink (K2EDTA)

Specimen Preparation

Maintain tubes at 37°C until separated from cells. Centrifuge samples to separate plasma from the red blood cells and place in ARUP standard transport tubes. Transport packed red blood cells (in original EDTA tubes) AND plasma (in ARUP standard transport tubes). (Min: 7 mL red blood cells and 10 mL plasma)

Storage/Transport Temperature


Unacceptable Conditions

Separator or gel tubes.


Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable


Qualitative Hemagglutination (HA)




2-5 days

Reference Interval

Interpretive Data

Compliance Category



Prior to ordering the thermal amplitude test, results from the Antibody ID Package (IRL) (ARUP test code 0013003) are required to identify specific antibodies that may interfere with testing. If Antibody ID Package has not been performed at ARUP within the last 7 days, the test will be added on by ARUP Laboratories and performed. Additional charges apply. Depending on antibody complexity, additional testing may be required. Additional charges apply. Client must provide patient transfusion history.

Hotline History


CPT Codes

86870; additional CPT codes may apply


Component Test Code* Component Chart Name LOINC
0013410 Thermal Amplitude Test
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Cold Autoantibody Evaluation
  • Thermal Amplitude
Thermal Amplitude Test