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Red Blood Cell Antigen Genotyping
3001053
Ordering Recommendation

Predict the expression of RBC antigen specificities to aid in selection of antigen-negative RBCs for transfusion, assess risk for hemolytic disease of the fetus and newborn, or resolve conflicting serological antibody results.

Mnemonic
RBC GENO
Methodology
Polymerase Chain Reaction/Fluorescence Monitoring
Performed
Sun-Sat
Reported
7-14 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) 
Specimen Preparation
Transport 3 mL whole blood. (Min: 1 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma or serum; collection of specimen in sodium heparin tubes. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Background Information for Red Blood Cell Antigen Genotyping:
Characteristics
: Erythrocyte alloimmunization may result in hemolytic transfusion reactions or hemolytic disease of the fetus and newborn (HDFN). Clinical presentation is variable and dependent upon the specific antibody and recipient factors.
Incidence: Erythrocyte alloimmunization occurs in up to 58% of sickle cell patients, up to 35% in other transfusion-dependent patients, and in approximately 0.8% of all pregnant women.
Inheritance: Typically co-dominant for red blood cell (RBC) antigens, autosomal recessive for hemoglobin S (HbS).
Cause: Antigen-antibody mediated red-cell hemolysis between donor/recipient or transferred maternal antibodies.
Variants Tested: See the "Additional Technical Information" document.
Clinical Sensitivity: >99% for c (RH4), C (RH2), e (RH5), E (RH3), k (KEL2), K (KEL1), Jka (JK1), Jkb (JK2), Fya (FY1), Fyb (FY2), M (MNS1), N (MNS2), S (MNS3), s (MNS4). Unknown for Kpa (KEL3), Kpb (KEL4), Jsa (KEL6), Jsb (KEL7), Lua (LU1), Lub (LU2), Dia (DI1), Dib (DI2), Coa (CO1), Cob (CO2), Doa (DO1), Dob (DO2), Joa (DO5), Hy (DO4), LWa (LW5), LWb (LW7), Sc1 (SC1), Sc2 (SC2), U (MNS5), V (RH10), VS (RH20), Hemoglobin S (HbS).
Methodology: Immucor PreciseType™ HEA Molecular BeadChip which is FDA-approved for clinical testing. Predicted phenotypes are reported for each antigen and HbS based on the variants tested.
Analytical Sensitivity and Specificity: >99% for c (RH4), C (RH2), e (RH5), E (RH3), k (KEL2), K (KEL1), Jka (JK1), Jkb (JK2), Fya (FY1), Fyb (FY2), M (MNS1), N (MNS2), S (MNS3), s (MNS4). Unknown for Kpa (KEL3), Kpb (KEL4), Jsa (KEL6), Jsb (KEL7), Lua (LU1), Lub (LU2), Dia (DI1), Dib (DI2), Coa (CO1), Cob (CO2), Doa (DO1), Dob (DO2), Joa (DO5), Hy (DO4), LWa (LW5), LWb (LW7), Sc1 (SC1), Sc2 (SC2), U (MNS5), V (RH10), VS (RH20), Hemoglobin S (HbS).
Limitations: Only the targeted variants will be interrogated. Rare nucleotide changes leading to altered or partial antigen expression and null phenotypes may not be detected by this assay. This assay does not assess for RhD nor is it designed to diagnose sickle cell disease. Patients who have had hematopoietic stem cell transplants may have inconclusive results on this test. Abnormal signal intensities may result in indeterminate genotyping results for all tested antigens/HbS.

Note
Hotline History
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Components
Component Test Code*Component Chart NameLOINC
3001054RBC Antigen Genotyping Specimen
3001055Rh Antigen C/c
3001056Rh Antigen E/e
3001057Rh Antigen V/VS
3001058Kell Antigen K/k
3001059Kell Antigen Kpa/Kpb
3001060Kell Antigen Jsa/Jsb
3001061Duffy Antigen Fya/Fyb
3001062Kidd Antigen Jka/Jkb
3001063MNS Antigen MN
3001064MNS Antigen S/s/U
3001065Lutheran Antigen Lua/Lub
3001066Dombrock Antigen Doa/Dob
3001067Dombrock Antigen Hy
3001068Dombrock Antigen Joa
3001069Landsteiner-Wiener Antigen LWa/LWb
3001070Diego Antigen Dia/Dib
3001071Colton Antigen Coa/Cob
3001072Scianna Antigen Sc1/Sc2
3001073Hemoglobin S Antigen
3001074RBC Antigen Genotyping Interpretation50398-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Colton genotyping (Coa, Cob)
  • Diego genotyping (Dia, Dib)
  • Dombrock genotyping (Doa, Dob, Hy, Joa)
  • Duffy genotyping (Fya, Fyb, Fyb-67C)
  • Kell genotyping (K, k, Kpa, Kpb, Jsa, Jsb)
  • Kidd genotyping (Jka, Jkb)
  • Landsteiner-Wiener genotyping (LWa, LWb)
  • Lutheran genotyping (Lua, Lub)
  • MNS genotyping (M, N, S, s, U, Uvar, Uneg)
  • Rh genotyping (C, c, E, e, V, VS)
  • Scianna genotyping (Sc1, Sc2)