QuantiFERON-TB Gold Plus, 1-Tube
Ordering Recommendation

Aid in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is less in low-risk populations. May be used in persons who have received BCG vaccine.

Cell Culture/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
QuantiFERON-TB Gold Plus 1-tube (ARUP Supply #54015) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. For collection and transport instructions refer to QuantiFERON under Special Handling at 
Specimen Preparation
Transport 6 mL whole blood. (Min: 5 mL). 
Storage/Transport Temperature
Refrigerated. Must be collected and shipped directly to ARUP the same calendar day
Unacceptable Conditions
Do not collect or ship on, or the day before, holidays. 
Ambient: 3 hours; Refrigerated: 48 hours; Frozen: Unacceptable 
Reference Interval
ComponentsReference Interval
QuantiFERON-TB Gold In-TubeNegative
QuantiFERON-TB1 minus NIL0.34 IU/mL or less
QuantiFERON-TB2 minus NIL0.34 IU/mL or less
QuantiFERON MITOGEN minus NILNo reference interval
QuantiFERON NILNo reference interval

Interpretive Data
Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection --- United States, 2010 (<>), for more information concerning test performance in low-prevalence populations and use in occupational screening.

If the stability requirements cannot be met, please refer to test 3000399, QuantiFERON-TB Gold Plus, 4-Tube.
Hotline History
View Hotline History
Component Test Code*Component Chart NameLOINC
2006962QuantiFERON Mitogen minus NIL71774-4
2006963QuantiFERON NIL71776-9
3000401Quantiferon TB Gold Plus71773-6
3000402Quantiferon Plus TB1 minus NIL64084-7
3000403Quantiferon Plus TB2 minus NIL88517-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Interferon-Gamma Release Assay for Tuberculosis
  • Latent Tuberculosis Infection (LTBI)
  • QFT & M TB
  • QFT-G & M. tuberculosis
  • QFT-TB; QFT-TB Plus
  • Quantiferon Mitogen and NIL
  • QuantiFERON-TB Gold In Tube
  • QuantiFERON-TB Gold Plus & Mycobacterium tuberculosis Antibody