Alpha Fetoprotein (Amniotic Fluid) with Reflex to Acetylcholinesterase and Fetal Hemoglobin
Ordering Recommendation

Evaluate possibility of a fetal open neural tube defect at 13-36 weeks of gestation.

Quantitative Chemiluminescent Immunoassay/Electrophoresis
3-4 days
Reflex: 3-11 days
New York DOH Approval Status
This test is New York DOH approved.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Amniocentesis. Specimen must be drawn between 13 weeks, 0 days and 36 weeks, 6 days gestation. 
Amniotic fluid. 
Specimen Preparation
Transport 2.5 mL amniotic fluid. (Min: 1.5 mL) 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Submit with Order: Gestational age at time of collection or estimated due date. 
Ambient: 1 month; Refrigerated: 3 months; Frozen: 3 months 
Reference Interval
Test Number
Reference Interval
 AFP, Amniotic FluidBy report
Ranges are based upon the weeks of gestation.
2006848Acetylcholinesterase and Fetal Hemoglobin, Amniotic FluidAcetylcholinesterase: Negative
Fetal Hemoglobin: Negative
 Multiple of Median1.99 or less

Interpretive Data
Refer to report.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Information must include weeks of gestation. If the AFP (amniotic fluid) is elevated, then Acetylcholinesterase will be added. Additional charges apply. Acetylcholinesterase testing requires an additional 3-11 days to be reported.
Hotline History
View Hotline History
CPT Code(s)
82106; if reflexed, add 82013 and 83033
Component Test Code*Component Chart NameLOINC
0080015AFP, Amniotic Fluid1832-5
0080929AFP AF Interpretation41273-4
3000162AFP, AF MoM29595-6
3000263Gestational Age Calculated at Collection18185-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • AFP
  • AFP-AF (Alpha-Fetoprotein, Amniotic Fluid)
  • Alpha Fetoprotein, Amniotic Fluid
  • Alpha-Fetoprotein, Amniotic Fluid
  • Fetoprotein, Amniotic Fluid