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Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental
2013107
Ordering Recommendation

Use only when patient has a repeatedly reactive third- or fourth-generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. 

Mnemonic
HIV AB SUP
Methodology
Qualitative Immunoassay
Performed
Varies
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), or Pink (K2 EDTA). Also acceptable: Serum Separator Tube (SST). 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma into an ARUP Standard Transport Tube dedicated only for HIV testing. (Min: 0.5 mL) Remove particulate material. 
Storage/Transport Temperature
Frozen. 
Unacceptable Conditions
Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
Remarks
 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Components
Reference Interval
HIV-1 AntibodyNegative
HIV-2 AntibodyNegative

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/P).

Note
For use ONLY when patient has a repeatedly reactive third- or fourth-generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. This test is for use as the antibody differentiation test in the specific multi-test algorithm. If results are negative or indeterminate, this test does NOT reflex to a nucleic acid test.

A multi-test algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to http://www.arupconsult.com/Topics/HIV.html).
Hotline History
N/A
CPT Code(s)
86701; 86702
Components
Component Test Code*Component Chart NameLOINC
2012670HIV-1/2 Ab Differentiation Immunoassay8251-1
2012671HIV-1 Antibody68961-2
2012672HIV-2 Antibody81641-3
2012673HIV Serologic Interpretation80203-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Geenius
  • HIV Diagnosis
  • HIV Multispot
  • HIV-1 & 2 Antibody Differentiation
  • HIV-1 and HIV-2 Antibody Differentiation
  • HIV-1 Antibody by Multispot
  • Human Immunodeficiency Virus 1/2 (HIV-1/2) Antibody, Differentiation