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Aggressive B-Cell Lymphoma FISH Reflex, Tissue
2012710
Ordering Recommendation

Aid in the diagnosis of aggressive large B-cell lymphoma with intermediate features between Burkitt lymphoma and diffuse large B-cell lymphoma (DLBCL) and confirmation of suspected double-hit lymphoma.

Mnemonic
DLBCL FISH
Methodology
Fluorescence in situ Hybridization
Performed
Varies
Reported
3-7 days, if reflexed, add 3 days for each reflex
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Tumor tissue. 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 8 unstained 3-micron slides. (Min: 4 slides) Protect paraffin block from excessive heat. Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months. 
Unacceptable Conditions
Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens. 
Remarks
Include surgical pathology report. 
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Note
If MYC (8q24) Gene Rearrangement by FISH is positive then IGH-BCL2 Fusion, t(14;18) by FISH will be added. If IGH-BCL2 Fusion, t(14;18) by FISH is negative then BCL6 (3q27) Gene Rearrangement by FISH will be added. Additional charges apply.
Hotline History
N/A
CPT Code(s)
88366, if reflexed add 88366; if further reflexed add 88366
Components
Component Test Code*Component Chart NameLOINC
2002346MYC by FISH
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 8q24 gene rearrangement FISH
  • Burkitt's Lymphoma
  • DLBCL
  • Double hit lymphoma, BCL6 FISH, BCL2 FISH
  • LSI MYC FISH
  • MYC FISH
  • MYC gene rearrangement FISH