Barbiturates, Urine, Quantitative
Ordering Recommendation

Preferred test to follow-up presumptive results. For general screening, Barbiturates, Urine Screen with Reflex to Quantitation (2012211) is preferred.

Quantitative Gas Chromatography-Mass Spectrometry
Tue, Thu, Sat
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Random urine. 
Specimen Preparation
Transfer 3.5 mL urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 1.5 mL) 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Specimens exposed to repeated freeze/thaw cycles. 
Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years 
Reference Interval
Drugs CoveredCutoff Concentrations
Butalbital50 ng/mL
Amobarbital50 ng/mL
Pentobarbital50 ng/mL
Secobarbital50 ng/mL
Phenobarbital50 ng/mL

Interpretive Data
Methodology: Quantitative Gas Chromatography-Mass Spectrometry.

Positive cutoff: 50 ng/mL

For medical purposes only; not valid for forensic use.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2012214Phenobarbital, Urn, Quant3950-3
2012215Butalbital, Urn, Quant11071-8
2012216Pentobarbital, Urn, Quant3926-3
2012217Amobarbital, Urn, Quant11230-0
2012218Secobarbital, Urn, Quant19695-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Amobarbital
  • Amobarbitone
  • Amytal
  • Axocet
  • Butalbital
  • Fioricet
  • Fiorinal
  • Luminal
  • Nembutal
  • Pain Management
  • Pain Management, Barbiturates, Quantitative, with medMATCH, Urine
  • Pain Management, Barbiturates, with Confirmation with medMATCH, Urine
  • Pentobarbital
  • Phenobarbital
  • Phenobarbitone
  • Quinalbartbitone
  • Sandoptal
  • Secobarbital
  • Seconal
  • Solfoton
  • Tuinal