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JAK2 Gene, V617F Mutation, Qualitative with Reflex to CALR (Calreticulin) Exon 9 Mutation Analysis by PCR with Reflex to MPL codon 515 Mutation Detection by Pyrosequencing, Quantitative
2012084
Ordering Recommendation

Use when diagnosis of essential thrombocytemia (ET) or primary myelofibrosis (PMF) is suspected.

Mnemonic
ET PMF RFX
Methodology
Polymerase Chain Reaction/Capillary Electrophoresis/Pyrosequencing
Performed
DNA Isolation: Sun-Sat
Assay: Mon, Wed, Fri
Reported
12-14 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) OR bone marrow (EDTA). 
Specimen Preparation
Do not freeze. Transport 5 mL whole blood. (Min: 1 mL) OR Transport 3 mL bone marrow. (Min: 1 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Serum. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens. 
Remarks
 
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
If JAK2 V617F is reported as "Not Detected" then CALR Exon 9 Mutation Analysis by PCR will be added. If CALR is reported as "Not Detected," then MPL codon 515 Mutation Detection by Pyrosequencing will be added. Additional charges apply.
CPT Code(s)
81270: if reflexed add 81219; if reflexed again add 81402
Components
Component Test Code*Component Chart NameLOINC
0051245JAK2 Gene, V617F Mutation, Qualitative43399-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases