CALR (Calreticulin) Exon 9 Mutation Analysis by PCR
2010673
Ordering Recommendation
Use for diagnostic and prognostic information in patients with myeloproliferative neoplasms (MPNs) when JAK2 testing is negative.
Mnemonic
CALR
Methodology
Polymerase Chain Reaction/Capillary Electrophoresis
Performed
DNA isolation: Sun-Sat
Assay: Sun, Tue-Fri
Assay: Sun, Tue-Fri
Reported
2-7 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
- Patient Preparation
- Collect
- Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.
- Specimen Preparation
- Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. - Storage/Transport Temperature
- Refrigerated.
- Unacceptable Conditions
- Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.
- Remarks
- Stability
- Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Reference Interval
Interpretive Data
Refer to report.
Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Note
Hotline History
View Hotline History
CPT Code(s)
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2010675 | CALR Exon 9 Mutation Analysis - Result | 35474-6 |
Aliases
- CALR
- ET
- JAK2-negative myeloproliferative neoplasm
- MPD
- MPL
- MPN
- Myeloproliferative disorders
- PMF