Human Immunodeficiency Virus Types 1 and 2 (HIV-1/HIV-2) Antibody Differentiation by Multispot (Supplemental Use Only)
2009464
Ordering Recommendation
Useful as a supplemental assay for repeatedly reactive third- or fourth-generation HIV screen tests, when following the CDC Proposed HIV Diagnostic Algorithm. This test cannot be used as a rapid screen nor as a follow-up for a positive rapid screen.
Submit With Order
Mnemonic
HIV MS
Methodology
Qualitative Immunoassay
Performed
Varies
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. Also acceptable: lavender (EDTA), or pink (K2EDTA)  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma into an ARUP Standard Transport Tube. (Min: 0.5 mL) Remove particulate material.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.  
Remarks
 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)  
Reference Interval
Available Separately Components Reference Interval
No HIV-​1 Antibody by Multispot Negative
No HIV-​2 Antibody by Multispot Negative
Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).
Note
For use only when patient has a repeatedly reactive third or fourth generation HIV screen test result. This test is for use as the antibody differentiation test in the specific multi-test algorithm proposed by the Centers for Disease Control and Prevention (CDC) and adopted by Clinical and Laboratory Standards Institute (CLSI) outlined here (refer to http://www.arupconsult.com/Algorithms/NewCDC_HIV_Alg.pdf). It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.

This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. A recommendation to order further testing on a separate specimen for HIV-1 Nucleic Acid will be made for certain results.
CPT Code(s)
86701; 86702
Components
Component Test Code*Component Chart Name
2007982HIV-1/HIV-2 Antibody Differentiation
2007984_HIV-1 Antibody by Multispot
2007985_HIV-2 Antibody by Multispot
2007986HIV Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1