Human Immunodeficiency Virus Types 1 and 2 (HIV-1/HIV-2) Antibody Differentiation by Multispot (Supplemental Use Only) INACTIVE as of 01/04/16: Contact laboratory for replacement test options)
Ordering Recommendation
Qualitative Immunoassay
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. Also acceptable: lavender (EDTA), or pink (K2EDTA) 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma into an ARUP Standard Transport Tube. (Min: 0.5 mL) Remove particulate material. 
Storage/Transport Temperature
Unacceptable Conditions
Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles) 
Reference Interval
Available SeparatelyComponentsReference Interval
NoHIV-1 Antibody by MultispotNegative
NoHIV-2 Antibody by MultispotNegative

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

For use only when patient has a repeatedly reactive third or fourth generation HIV screen test result. This test is for use as the antibody differentiation test in the specific multi-test algorithm proposed by the Centers for Disease Control and Prevention (CDC) and adopted by Clinical and Laboratory Standards Institute (CLSI) outlined here (refer to It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.

This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported.  A recommendation to order further testing on a separate specimen for HIV-1 Nucleic Acid will be made for certain results.
CPT Code(s)
86701; 86702
Component Test Code*Component Chart NameLOINC
2007982HIV-1/HIV-2 Antibody Differentiation42768-2
2007984_HIV-1 Antibody by Multispot29893-5
2007985_HIV-2 Antibody by Multispot30361-0
2007986HIV Interpretation69668-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1