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Human Immunodeficiency Virus Types 1 and 2 (HIV-1/HIV-2) Antibody Differentiation by Multispot (Supplemental Use Only) INACTIVE as of 01/04/16: Contact laboratory for replacement test options)
2009464
Ordering Recommendation
Mnemonic
HIV MS
Methodology
Qualitative Immunoassay
Performed
Varies
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. Also acceptable: lavender (EDTA), or pink (K2EDTA) 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma into an ARUP Standard Transport Tube. (Min: 0.5 mL) Remove particulate material. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
Remarks
 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles) 
Reference Interval
Available SeparatelyComponentsReference Interval
NoHIV-1 Antibody by MultispotNegative
NoHIV-2 Antibody by MultispotNegative

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Note
For use only when patient has a repeatedly reactive third or fourth generation HIV screen test result. This test is for use as the antibody differentiation test in the specific multi-test algorithm proposed by the Centers for Disease Control and Prevention (CDC) and adopted by Clinical and Laboratory Standards Institute (CLSI) outlined here (refer to http://www.arupconsult.com/Algorithms/NewCDC_HIV_Alg.pdf). It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.

This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported.  A recommendation to order further testing on a separate specimen for HIV-1 Nucleic Acid will be made for certain results.
CPT Code(s)
86701; 86702
Components
Component Test Code*Component Chart NameLOINC
2007982HIV-1/HIV-2 Antibody Differentiation42768-2
2007984_HIV-1 Antibody by Multispot29893-5
2007985_HIV-2 Antibody by Multispot30361-0
2007986HIV Interpretation69668-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1