MGMT Methylation Detection by PCR
2009310
Ordering Recommendation
Aids in therapeutic decisions in individuals with gliomas. Methylation of MGMT gene is a favorable prognostic factor in glial tumors.
Mnemonic
MGMT
Methodology
Real-Time Polymerase Chain Reaction/Fluorescence Resonance Energy Transfer
Performed
DNA isolation: Sun-Sat
Assay: Varies
Reported
10-12 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Ship in cooled container during summer months.  
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens. Less than 25 percent tumor.  
Remarks
Include surgical pathology report. Tissue block will be returned after testing.  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
   
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
 
CPT Code(s)
88381, 81287
Components
Component Test Code*Component Chart NameLOINC
2002148Block ID57723-9
2009311MGMT Methylation Detection - Results 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases