MGMT Methylation Detection by PCR
Ordering Recommendation
Aids in therapeutic decisions in individuals with gliomas. Methylation of MGMT gene is a favorable prognostic factor in glial tumors.
Submit With Order
Real-Time Polymerase Chain Reaction/Fluorescence Resonance Energy Transfer
DNA isolation: Sun-Sat
Assay: Varies
10-12 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Ship in cooled container during summer months.  
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens. Less than 25 percent tumor.  
Include surgical pathology report. Tissue block will be returned after testing.  
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

See Compliance Statement B:
CPT Code(s)
88381, 81287
Component Test Code*Component Chart Name
2002148Block ID
2009311MGMT Methylation Detection - Results
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References