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Pneumocystis jirovecii DFA with Reflex to Pneumocystis jirovecii by PCR
2009226
Ordering Recommendation

Use for rapid identification of P. jirovecii. Negative DFA results are confirmed by PCR testing. Sensitivity is dependent on patient population and specimen type.

Mnemonic
PNEUMST R
Methodology
Direct Fluorescent Antibody Stain/Qualitative Polymerase Chain Reaction
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Respiratory specimen: Bronchial washing, bronchoalveolar lavage (BAL), or induced sputum. 
Specimen Preparation
Transfer 5 mL respiratory specimen to a sterile container. (Min: 1 mL)  Place each specimen in an individually sealed bag. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Tissues. Formalinized specimens. Slides or swabs. 
Remarks
Specimen source required. 
Stability
Ambient: 2 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
Negative
Interpretive Data


Note
A negative stain result does not exclude the possibility of infection. False-negative results may occur due to sampling errors or a low number of organisms in the specimen. If Pneumocystis jirovecii DFA is negative, then Pneumocystis jirovecii by PCR testing will be added. Additional charges apply.
CPT Code(s)
87299; if reflexed add 87798
Components
Component Test Code*Component Chart NameLOINC
2002588Pneumocystis source:31208-2
2009227Pneumocystis jirovecii - DFA700-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases