B-Cell Memory and Naive Panel
Ordering Recommendation

Assess B-cell subsets of immunodeficiencies. Supports the diagnosis of common variable immune deficiency (CVID) and may help predict the clinical phenotype. Assess B-cell reconstitution after bone marrow or hematopoietic stem cell transplantation. Not recommended for rituximab monitoring; refer to B-Cell CD20 Expression (0092099).

Flow Cytometry
1-3 days
1-3 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin). 
Specimen Preparation
Transport 4 mL whole blood. (Min: 0.5 mL) 
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. 
Unacceptable Conditions
Specimens older than 48 hours. Clotted or hemolyzed specimens. 
Specimens must be analyzed within 48 hours of collection. 
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable 
Reference Interval
Effective May 16, 2016
Available SeparatelyComponentsReference Interval
2-5 months5-9 months9-15 months15-24 months2-5 years5-10 years10-16 years16 years and older
NoB-cells % CD1918-38 %16-34 %14-28 %16-34 %14-29 %10-24 %9-23 %5-26 %
NoB-cells Absolute CD19700-2400 cells/µL700-2800 cells/µL400-2900 cells/µL600-1900 cells/µL400-1700 cells/µL300-600 cells/µL200-600 cells/µL58-558 cells/µL
NoNaive B-cell % CD19+/CD27-/IgD+82-95 %86-93 %77-95 %68-89 %54-88 %47-77 %51-83 %29-93 %
NoNaive B-cell Absolute CD19+/CD27-/IgD+620-2120 cells/µL600-2590 cells/µL360-2800 cells/µL490-1560 cells/µL280-1330 cells/µL130-460 cells/µL120-430 cells/µL22-423 cells/µL
NoClass-switched Mem%CD19+/CD27+/IgD-/IgM-0-9 %2-7 %1-12 %4-14 %5-21 %11-30 %9-26 %3-23 %
NoClass-switched Abs CD19+/CD27+/IgD-/IgM-10-170 cells/µL20-140 cells/µL10-100 cells/µL30-180 cells/µL20-220 cells/µL40-140 cells/µL30-110 cells/µL4-62 cells/µL
NoNon-Switched Mem %CD19+/CD27+/IgD+/IgM+3-9 %3-7 %3-11 %4-14 %3-20 %5-20 %5-18 %2-25 %
NoNon-switched Abs CD19+/CD27+/IgD+/IgM+20-200 cells/µL30-120 cells/µL20-140 cells/µL30-170 cells/µL20-180 cells/µL20-100 cells/µL20-70 cells/µL4-66 cells/µL
NoTotal Memory B-cell % CD19+/CD27+3-12 %5-12 %4-21 %10-27 %8-37 %19-47 %13-48 %7-48 %
NoTotal Memory B-cell Absolute CD19+/CD27+40-230 cells/µL50-270 cells/µL40-190 cells/µL50-330 cells/µL50-390 cells/µL60-230 cells/µL50-200 cells/µL13-148 cells/µL
NoIgM Only Memory Pct CD19+CD27+1gD-IgM+0.3-6.0 %0.3-6.0 %0.3-6.0 %0.3-6.0 %0.3-6.0 %0.3-6.0 %0.3-6.0 %0.3-6.0 %
NoIgM Only Memory Abs CD19+CD27+IgD-IgM+0.6-16.4 cells/µL0.6-16.4 cells/µL0.6-16.4 cells/µL0.6-16.4 cells/µL0.6-16.4 cells/µL0.6-16.4 cells/µL0.6-16.4 cells/µL0.6-16.4 cells/µL

Interpretive Data
This panel is indicated for patients with suspected immune deficiencies, especially Common Variable Immune Deficiency (CVID), and to assess reconstitution of B-cell subsets after bone marrow or stem cell transplant. Subsets measured: B-cells (CD19+), total memory B-cells (CD19+ CD27+), class switched memory B-cells (CD19+ CD27+ IgD- IgM-), non-switched/marginal zone memory B-cells (CD19+ CD27+ IgD+ IgM+), IgM only memory B-cells (CD19+ CD27+ IgD-IgM+), and naive B-cells (CD19+ CD27-IgD+).

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Reference intervals for IgM-only memory B-cells have currently been established only for populations aged 16-years and older. For all other B-cell subsets, reference intervals for populations younger than 16-years are adopted from literature. Piatosa B, Wolska-Kusnierz B, Pac M, Siewiera K, Galkowska E, Bernatowska E. B cell subsets in healthy children: Reference values for evaluation of B cell maturation process in peripheral blood. Cytometry Part B 2010; 78B: 372381.
Hotline History
Component Test Code*Component Chart NameLOINC
2008902B-cells % CD198117-4
2008903B-cells Absolute CD198116-6
2008904Class-switched Mem%CD19+/CD27+/IgD-/IgM-
2008905Class-switched Abs CD19+/CD27+/IgD-/IgM-
2008906Non-Switched Mem %CD19+/CD27+/IgD+/IgM+43273-2
2008907Non-Switched Abs CD19+/CD27+/IgD+/IgM+
2008908Naive B-cell % CD19+/CD27-/IgD+
2008909Naive B-cell Absolute CD19+/CD27-/IgD+
2008910Total Memory B-cell % CD19+/CD27+
2008911Total Memory B-cell Absolute CD19+/CD27+
2013303IgM Only Memory Abs CD19+CD27+IgD-IgM+
2013304IgM Only Memory Pct CD19+CD27+IgD-IgM+
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Memory B-cells