B-Cell Memory and Naive Panel
2008901
 
Ordering Recommendation
Assess B-cell subsets in immunodeficiencies, such as common variable immunodeficiency or B-cell reconstitution after bone marrow or hematopoietic stem cell transplantation.
Mnemonic
CVID
Methodology
Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days
1-3 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).  
Specimen Preparation
Transport 4 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated.  
Unacceptable Conditions
Specimens older than 48 hours. Clotted or hemolyzed specimens.  
Remarks
Specimens must be analyzed within 48 hours of collection.  
Stability
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable  
Reference Interval
Available Separately Components Reference Interval
2-​5 months 5-​9 months 9-​15 months 15-​24 months 2-​5 years 5-​10 years 10-​16 years 16 years & older
No B-​cells % CD19 18-​38 % 16-​34 % 14-​28 % 16-​34 % 14-​29 % 10-​24 % 9-​23 % 6-​28 %
No B-​cells Absolute CD19 700-​2400 cells/µL 700-​2800 cells/µL 400-​2900 cells/µL 600-​1900 cells/µL 400-​1700 cells/µL 300-​600 cells/µL 200-​600 cells/µL 94-​588 cells/µL
No Naive B-​cell % CD19+/CD27-​/IgD+ 82-​95 % 86-​93 % 77-​95 % 68-​89 % 54-​88 % 47-​77 % 51-​83 % 0-​100 %
No Naive B-​cell Absolute CD19+/CD27-​/IgD+ 620-​2120 cells/µL 600-​2590 cells/µL 360-​2800 cells/µL 490-​1560 cells/µL 280-​1330 cells/µL 130-​460 cells/µL 120-​430 cells/µL 5-​345 cells/µL
No Class-​switched Memory % CD19+/CD27+/IgD-​ 0-​9 % 2-​7 % 1-​12 % 4-​14 % 5-​21 % 11-​30 % 9-​26 % 4-​40 %
No Class-​switched Absolute CD19+/CD27+/IgD-​ 10-​170 cells/µL 20-​140 cells/µL 10-​100 cells/µL 30-​180 cells/µL 20-​220 cells/µL 40-​140 cells/µL 30-​110 cells/µL 7-​155 cells/µL
No Non-​switched Memory % CD19+/CD27+/IgD+ 3-​9 % 3-​7 % 3-​11 % 4-​14 % 3-​20 % 5-​20 % 5-​18 % 3-​35 %
No Non-​switched Absolute CD19+/CD27+/IgD+ 20-​200 cells/µL 30-​120 cells/µL 20-​140 cells/µL 30-​170 cells/µL 20-​180 cells/µL 20-​100 cells/µL 20-​70 cells/µL 5-​100 cells/µL
No Total Memory B-​cell % CD19+/CD27+ 3-​12 % 5-​12 % 4-​21 % 10-​27 % 8-​37 % 19-​47 % 13-​48 % 9-​64 %
No Total Memory B-​cell Abs CD19+/CD27+ 40-​230 cells/µL 50-​270 cells/µL 40-​190 cells/µL 50-​330 cells/µL 50-​390 cells/µL 60-​230 cells/µL 50-​200 cells/µL 18-​242 cells/µL
Interpretive Data
This test measures B-cells (CD19+), total memory B-cells (CD19+ CD27+), class switched memory B-cells (CD19+ CD27+ IgD-), non-switched/marginal zone memory B-cells (CD19+ CD27+ IgD+), and naive B-cells (CD19+ CD27- IgD+). This test is indicated for patients with suspected immune deficiencies, especially Common Variable Immune Deficiency (CVID), and to assess reconstitution of B-cell subsets after bone marrow or stem cell transplant.

See Compliance Statement A: www.aruplab.com/CS
Note
CPT Code(s)
86355; 86356 x2
Components
Component Test Code*Component Chart Name
2008902B-cells % CD19
2008903B-cells Absolute CD19
2008904Class-switched Memory %CD19+/CD27+/IgD-
2008905Class-switched Absolute CD19+/CD27+/IgD-
2008906Non-Switched Memory %CD19+/CD27+/IgD+
2008907Non-switched Absolute CD19+/CD27+/IgD+
2008908Naive B-cell % CD19+/CD27-/IgD+
2008909Naive B-cell Absolute CD19+/CD27-/IgD+
2008910Total Memory B-cell % CD19+/CD27+
2008911Total Memory B-cell Absolute CD19+/CD27+
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Memory B-cells