EGFR Gene Amplification by FISH
Ordering Recommendation
Aids in prognostication and therapeutic decisions for neoplasms where amplification has been demonstrated.
Fluorescence in situ Hybridization
4-12 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 6 unstained 5 micron slides. (Min: 3 slides) Protect paraffin block from excessive heat. Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Frozen speicmens. Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.  
Include surgical pathology report  
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Components Reference Interval
EGFR by FISH Nonamplified: LSI EGFR:CEP7 Less than 2.0
Amplified: LSI EGFR:CEP7 Greater than or equal to 2.0
EGFR:CEP7 By report
EGFR per Cells Counted By report
Interpretive Data
Refer to report.

See Compliance Statement A:
Statement A: Analyte specific reagents (ASR) are used in many laboratory tests necessary for standard medical care and generally do not require U.S. Food and Drug Administration (FDA) approval or clearance. This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. This test should not be regarded as investigational or for research use.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0049242EGFR by FISH 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • EGFR
  • EGFR (Her-1)
  • EGFR amplification by FISH
  • Epidermal Growth Factor Receptor
  • erbB-1
  • Her-1