EGFR Gene Amplification by FISH
2008605
Ordering Recommendation
Aids in prognostication and therapeutic decisions for neoplasms where amplification has been demonstrated.
Submit With Order
Mnemonic
EGFR FISH
Methodology
Fluorescence in situ Hybridization
Performed
Varies
Reported
4-12 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 6 unstained 5 micron slides. (Min: 3 slides) Protect paraffin block from excessive heat. Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Frozen speicmens. Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.  
Remarks
Include surgical pathology report  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Components Reference Interval
EGFR by FISH Nonamplified: LSI EGFR:CEP7 Less than 2.0
Amplified: LSI EGFR:CEP7 Greater than or equal to 2.0
EGFR:CEP7 By report
EGFR per Cells Counted By report
Interpretive Data
Refer to report.

See Compliance Statement A: www.aruplab.com/CS
Note
CPT Code(s)
88367
Components
Component Test Code*Component Chart Name
0049242EGFR by FISH
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • EGFR
  • EGFR (Her-1)
  • EGFR amplification by FISH
  • EGFR FISH
  • Epidermal Growth Factor Receptor
  • erbB-1
  • Her-1
  • HER1/EGFR